cDNA Clone MEKK3 (mouse)

• Synonyms: Map3k3
• Plasmid: pCMV5-HA3 (mouse) MEKK3 K391M
• Parent Plasmid: pCMV5 HA3
• Expressed: HA-MEKK3 K391M
• Genbank: NM_011947
• Tag: HA
• Species: Mouse

Sequence of Insert

GTCGACATGGATGAACAAGAGGCATTAGACTCGATCATGAAGGACCTGGTGGCCCTCCAGATGAGCCGACGAACCCGGTTGTCTGGATATGAGACCATGAAGAATAAGGACACAGGTCACCCAAACAGGCAGAGTGACGTCAGAATCAAGTTTGAACACAATGGGGAGAGACGAATTATAGCATTCAGCCGGCCTGTGAGATACGAAGATGTGGAGCACAAGGTGACAACAGTCTTTGGGCAGCCTCTTGATTTGCATTATATGAATAATGAGCTCTCCATCCTGTTGAAAAACCAAGATGATCTC
GATAAAGCCATTGACATTTTGGATAGAAGCTCAAGTATGAAAAGCCTTAG
GATACTACTGTTATCCCAAGACAGAAACCATACTAGTTCCTCTCCCCACT
CTGGAGTGTCCAGGCAGGTTCGGATCAAGCCTTCCCAGTCTGCAGGGGAT
ATAAATACCATCTACCAAGCTCCTGAGCCCAGAAGCAGGCACCTGTCTGT
CAGCTCCCAGAACCCTGGCCGAAGCTCTCCTCCCCCGGGATATGTACCTG
AGCGACAACAGCACATTGCCCGGCAAGGATCCTATACGAGCATCAACAGC
GAAGGTGAATTCATCCCAGAGACCAGCGAACAGTGTATGCTAGATCCCCT
CAGCAGTGCCGAAAATTCCTTGTCAGGAAGCTGCCAATCCTTGGACAGGT
CAGCAGACAGCCCATCCTTCAGGAAATCACAAATGTCCCGAGCCCGGAGC
TTCCCAGACAACAGAAAGGAATGCTCAGATCGGGAGACCCAGCTCTATGA
TAAAGGTGTCAAAGGTGGAACCTATCCCAGGCGCTACCATGTGTCTGTGC
ATCACAAAGACTACAATGATGGCAGAAGAACATTTCCCCGAATACGACGG
CATCAAGGCAACCTATTCACTCTGGTGCCCTCAAGTCGCTCCTTGAGCAC
AAATGGCGAGAACATGGGTGTAGCTGTGCAATACCTGGACCCCCGTGGGC
GCCTACGGAGTGCAGACAGTGAGAATGCCCTCACTGTGCAGGAAAGGAAT
GTGCCAACCAAATCTCCTAGTGCTCCCATCAATTGGCGTCGGGGGAAGCT
CCTGGGTCAAGGTGCCTTCGGCAGGGTCTACTTGTGCTATGATGTGGACA
CAGGACGTGAACTTGCTTCTATGCAGGTCCAGTTTGACCCAGATAGTCCT
GAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGTGAGATCCAGTTGCTGAA
GAACCTGCAGCATGAGCGCATTGTGCAGTACTACGGCTGCCTGCGGGACC
GTGCTGAGAAGATCCTCACCATCTTTATGGAGTATATGCCAGGGGGCTCT
GTAAAAGACCAGTTGAAGGCCTACGGAGCTCTGACAGAGAGTGTGACCCG
CAAGTACACCCGGCAGATTCTGGAGGGCATGTCATACCTGCACAGCAACA
TGATTGTGCATCGGGACATCAAGGGAGCCAATATCCTCCGAGACTCAGCT
GGGAATGTGAAGCTTGGGGATTTTGGGGCCAGCAAACGCCTACAGACCAT
CTGCATGTCAGGGACAGGCATTCGCTCTGTCACTGGCACACCCTACTGGA
TGAGTCCTGAAGTCATCAGTGGCGAGGGCTATGGAAGAAAGGCAGACGTG
TGGAGCCTGGGCTGTACTGTGGTGGAAATGCTGACAGAGAAACCACCTTG
GGCAGAGTATGAAGCTATGGCTGCCATTTTCAAGATTGCCACCCAGCCTA
CCAATCCTCAGCTGCCCTCTCACATCTCAGAACACGGCAGGGACTTCCTG
AGGCGCATATTTGTGGAAGCTCGTCAGAGACCCTCAGCTGAGGAGCTGCT
CACACACCACTTTGCACAGCTAGTGTACTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMDEQEALDSIMKDLVALQMSRRTRLSGYETM
KNKDTGHPNRQSDVRIKFEHNGERRIIAFSRPVRYEDVEHKVTTVFGQPL
DLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHTSSS
PHSGVSRQVRIKPSQSAGDINTIYQAPEPRSRHLSVSSQNPGRSSPPPGY
VPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSL
DRSADSPSFRKSQMSRARSFPDNRKECSDRETQLYDKGVKGGTYPRRYHV
SVHHKDYNDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGVAVQYLDP
RGRLRSADSENALTVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYD
VDTGRELASMQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCL
RDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLH
SNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTP
YWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIAT
QPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLVY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5639
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 72989 Daltons