cDNA Clone MEKK3 (mouse)

• Synonyms: Map3k3
• Plasmid: pEBG6P-1 (mouse) MEKK3 K391M
• Parent Plasmid: pEBG6P
• Expressed: GST-MEKK3 K391M
• Genbank: NM_011947
• Tag: GST
• Species: Mouse

Sequence of Insert

GGTACCATGGATGAACAAGAGGCATTAGACTCGATCATGAAGGACCTGGTGGCCCTCCAGATGAGCCGACGAACCCGGTTGTCTGGATATGAGACCATGAAGAATAAGGACACAGGTCACCCAAACAGGCAGAGTGACGTCAGAATCAAGTTTGAACACAATGGGGAGAGACGAATTATAGCATTCAGCCGGCCTGTGAGATACGAAGATGTGGAGCACAAGGTGACAACAGTCTTTGGGCAGCCTCTTGATTTGCATTATATGAATAATGAGCTCTCCATCCTGTTGAAAAACCAAGATGATCTC
GATAAAGCCATTGACATTTTGGATAGAAGCTCAAGTATGAAAAGCCTTAG
GATACTACTGTTATCCCAAGACAGAAACCATACTAGTTCCTCTCCCCACT
CTGGAGTGTCCAGGCAGGTTCGGATCAAGCCTTCCCAGTCTGCAGGGGAT
ATAAATACCATCTACCAAGCTCCTGAGCCCAGAAGCAGGCACCTGTCTGT
CAGCTCCCAGAACCCTGGCCGAAGCTCTCCTCCCCCGGGATATGTACCTG
AGCGACAACAGCACATTGCCCGGCAAGGATCCTATACGAGCATCAACAGC
GAAGGTGAATTCATCCCAGAGACCAGCGAACAGTGTATGCTAGATCCCCT
CAGCAGTGCCGAAAATTCCTTGTCAGGAAGCTGCCAATCCTTGGACAGGT
CAGCAGACAGCCCATCCTTCAGGAAATCACAAATGTCCCGAGCCCGGAGC
TTCCCAGACAACAGAAAGGAATGCTCAGATCGGGAGACCCAGCTCTATGA
TAAAGGTGTCAAAGGTGGAACCTATCCCAGGCGCTACCATGTGTCTGTGC
ATCACAAAGACTACAATGATGGCAGAAGAACATTTCCCCGAATACGACGG
CATCAAGGCAACCTATTCACTCTGGTGCCCTCAAGTCGCTCCTTGAGCAC
AAATGGCGAGAACATGGGTGTAGCTGTGCAATACCTGGACCCCCGTGGGC
GCCTACGGAGTGCAGACAGTGAGAATGCCCTCACTGTGCAGGAAAGGAAT
GTGCCAACCAAATCTCCTAGTGCTCCCATCAATTGGCGTCGGGGGAAGCT
CCTGGGTCAAGGTGCCTTCGGCAGGGTCTACTTGTGCTATGATGTGGACA
CAGGACGTGAACTTGCTTCTATGCAGGTCCAGTTTGACCCAGATAGTCCT
GAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGTGAGATCCAGTTGCTGAA
GAACCTGCAGCATGAGCGCATTGTGCAGTACTACGGCTGCCTGCGGGACC
GTGCTGAGAAGATCCTCACCATCTTTATGGAGTATATGCCAGGGGGCTCT
GTAAAAGACCAGTTGAAGGCCTACGGAGCTCTGACAGAGAGTGTGACCCG
CAAGTACACCCGGCAGATTCTGGAGGGCATGTCATACCTGCACAGCAACA
TGATTGTGCATCGGGACATCAAGGGAGCCAATATCCTCCGAGACTCAGCT
GGGAATGTGAAGCTTGGGGATTTTGGGGCCAGCAAACGCCTACAGACCAT
CTGCATGTCAGGGACAGGCATTCGCTCTGTCACTGGCACACCCTACTGGA
TGAGTCCTGAAGTCATCAGTGGCGAGGGCTATGGAAGAAAGGCAGACGTG
TGGAGCCTGGGCTGTACTGTGGTGGAAATGCTGACAGAGAAACCACCTTG
GGCAGAGTATGAAGCTATGGCTGCCATTTTCAAGATTGCCACCCAGCCTA
CCAATCCTCAGCTGCCCTCTCACATCTCAGAACACGGCAGGGACTTCCTG
AGGCGCATATTTGTGGAAGCTCGTCAGAGACCCTCAGCTGAGGAGCTGCT
CACACACCACTTTGCACAGCTAGTGTACTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMDEQEALDS
IMKDLVALQMSRRTRLSGYETMKNKDTGHPNRQSDVRIKFEHNGERRIIA
FSRPVRYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDR
SSSMKSLRILLLSQDRNHTSSSPHSGVSRQVRIKPSQSAGDINTIYQAPE
PRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETS
EQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSQMSRARSFPDNRKECS
DRETQLYDKGVKGGTYPRRYHVSVHHKDYNDGRRTFPRIRRHQGNLFTLV
PSSRSLSTNGENMGVAVQYLDPRGRLRSADSENALTVQERNVPTKSPSAP
INWRRGKLLGQGAFGRVYLCYDVDTGRELASMQVQFDPDSPETSKEVSAL
ECEIQLLKNLQHERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYG
ALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFG
ASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVE
MLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQ
RPSAEELLTHHFAQLVY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5638
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mouse MEKK3 K391M with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 98537 Daltons