cDNA Clone NUAK1

• Plasmid: pEBG-2T-NUAK1 I300-Y310
• Parent Plasmid: pEBG2T
• Expressed: GST-NUAK1 I300-Y310
• Genbank: NM_014840
• Tag: GST
• Species: Human

Sequence of Insert

ACTAGTATTGCCAACCACTGGTGGGTGAACTGGGGCTATTAGAGCAGCGTGTGTGACTGTGATGCCCTCCATGACTCTGAGTCCCCACTCCTGGCTCGGATCATTGACTGGCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCCAAAATGAAGGGCCTGGCCAAACCCACGACCTCTGAGGTCATGCTAGAGCGGCAGCGGTCGCTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCTGGTCAGGATGCAGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCCAAGGGG
ATCCTGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCACTGGCTT
CATTGAAGGTGTAGTTGGTCCTGCCTTACCCTCTACTTTCAAGATGGAGC
AGGACTTGTGCAGGACTGGCGTGCTCCTCCCAAGCTCACCAGAGGCAGAG
GTGCCGGGAAAACTCAGCCCCAAGCAGTCGGCCACGATGCCCAAGAAAGG
CATCTTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCTTCCCCAG
AGCGCAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATGGGCAGC
AGCATCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTCCCACAG
CCTCTCCTGCCGGAGGAAGGGCATCTTGAAACACAGCAGCAAATACTCAG
CGGGCACCATGGACCCAGCCCTGGTCAGCCCTGAAATGCCCACACTGGAA
TCCCTGTCAGAGCCTGGTGTCCCTGCCGAGGGCCTCTCCCGGAGCTACAG
CCGCCCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGCGACTCTT
TTGACTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGCATCCGC
AGCTGCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGAGGGGCT
CCAGAACCGGCCCCGGCCCCAGTACCTGAAGCGGTACC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSIANHWWVNWGY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5620
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express NUAK1 I300-Y310 with N-terminal GST.; ; Estimated MW of expressed protein : 27909 Daltons