cDNA Clone NUAK1

• Plasmid: pEBG-2T-NUAK1 T250-Y310
• Parent Plasmid: pEBG2T
• Expressed: GST-NUAK1 T250-Y310
• Genbank: NM_014840
• Tag: GST
• Species: Human

Sequence of Insert

ACTAGTACAATGCCCTTCGATGGTTTCGATCACAAAAACCTCATTCGGCA
AATCAGCAGCGGAGAGTACCGGGAGCCAACACAGCCCTCAGATGCTCGAG
GACTCATACGGTGGATGCTGATGGTGAACCCCGATCGCCGGGCCACTATT
GAGGACATTGCCAACCACTGGTGGGTGAACTGGGGCTATTAGAGCAGCGT
GTGTGACTGTGATGCCCTCCATGACTCTGAGTCCCCACTCCTGGCTCGGA
TCATTGACTGGCACCACCGTTCCACAGGGCTGCAGGCTGACACCGAAGCC
AAAATGAAGGGCCTGGCCAAACCCACGACCTCTGAGGTCATGCTAGAGCG
GCAGCGGTCGCTGAAGAAATCCAAGAAAGAGAATGACTTTGCTCAGTCTG
GTCAGGATGCAGTGCCTGAAAGCCCATCCAAGTTGAGTTCTAAGAGGCCC
AAGGGGATCCTGAAGAAGCGAAGCAACAGCGAGCATCGCTCTCACAGCAC
TGGCTTCATTGAAGGTGTAGTTGGTCCTGCCTTACCCTCTACTTTCAAGA
TGGAGCAGGACTTGTGCAGGACTGGCGTGCTCCTCCCAAGCTCACCAGAG
GCAGAGGTGCCGGGAAAACTCAGCCCCAAGCAGTCGGCCACGATGCCCAA
GAAAGGCATCTTGAAAAAGACCCAGCAGAGAGAATCAGGTTACTACTCTT
CCCCAGAGCGCAGTGAGTCTTCGGAGCTGTTGGACAGTAATGATGTGATG
GGCAGCAGCATCCCCTCCCCCAGCCCCCCGGACCCAGCCAGGGTAACCTC
CCACAGCCTCTCCTGCCGGAGGAAGGGCATCTTGAAACACAGCAGCAAAT
ACTCAGCGGGCACCATGGACCCAGCCCTGGTCAGCCCTGAAATGCCCACA
CTGGAATCCCTGTCAGAGCCTGGTGTCCCTGCCGAGGGCCTCTCCCGGAG
CTACAGCCGCCCTTCCAGTGTCATCAGCGATGACAGCGTGCTGTCCAGCG
ACTCTTTTGACTTGCTGGATTTGCAGGAGAATCGCCCTGCCCGCCAGCGC
ATCCGCAGCTGCGTCTCTGCAGAAAACTTCCTCCAGATCCAGGACTTTGA
GGGGCTCCAGAACCGGCCCCGGCCCCAGTACCTGAAGCGGTACC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSTSTMPFDGFDHKNLIRQISSGEYR
EPTQPSDARGLIRWMLMVNPDRRATIEDIANHWWVNWGY*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express NUAK1 T250-Y310 with N-terminal GST.; ; Estimated MW of expressed protein : 33754 Daltons