cDNA Clone CK1 gamma

• Synonyms: CSNK1G1
• Plasmid: pGEX6P-3-CK1 Gamma (lost?)
• Parent Plasmid: pGEX6P3
• Expressed: GST-CK1 Gamma
• Genbank: NM_001011664
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGACCATCCTAGTAGGGAAAAGGATGAAAGACAACGGACAACTAAACCCATGGCACAAAGGAGTGCACACTGCTCTCGACCATCTGGCTCCTCATCGTCCTCTGGGGTTCTTATGGTGGGACCCAACTTCAGGGTTGGCAAGAAGATAGGATGTGGGAACTTCGGAGAGCTCAGATTAGGTAAAAATCTCTACACCAATGAATATGTAGCAATCAAACTGGAACCAATAAAATCACGTGCTCCACAGCTTCATTTAGAGTACAGATTTTATAAACAGCTTGGCAGTGCAGGTGAAGGT
CTCCCACAGGTGTATTACTTTGGACCATGTGGGAAATATAATGCCATGGT
GCTGGAGCTCCTTGGCCCTAGCTTGGAGGACTTGTTTGACCTCTGTGACC
GAACATTTACTTTGAAGACGGTGTTAATGATAGCCATCCAGCTGCTTTCT
CGAATGGAATACGTGCACTCAAAGAACCTCATTTACCGAGATGTCAAGCC
AGAGAACTTCCTGATTGGTCGACAAGGCAATAAGAAAGAGCATGTTATAC
ACATTATAGACTTTGGACTGGCCAAGGAATACATTGACCCCGAAACCAAA
AAACACATACCTTATAGGGAACACAAAAGTTTAACTGGAACTGCAAGATA
TATGTCTATCAACACGCATCTTGGCAAAGAGCAAAGCCGGAGAGATGATT
TGGAAGCCCTAGGCCACATGTTCATGTATTTCCTTCGAGGCAGCCTCCCC
TGGCAAGGACTCAAGGCTGACACATTAAAAGAGAGATATCAAAAAATTGG
TGACACCAAAAGGAATACTCCCATTGAAGCTCTCTGTGAGAACTTTCCAG
AGGAGATGGCAACCTACCTTCGATATGTCAGGCGACTGGACTTCTTTGAA
AAACCTGATTATGAGTATTTACGGACCCTCTTCACAGACCTCTTTGAAAA
GAAAGGCTACACCTTTGACTATGCCTATGATTGGGTTGGGAGACCTATTC
CTACTCCAGTAGGGTCAGTTCACGTAGATTCTGGTGCATCTGCAATAACT
CGAGAAAGCCACACACATAGGGATCGGCCATCACAACAGCAGCCTCTTCG
AAATCAGGTGGTTAGCTCAACCAATGGAGAGCTGAATGTTGATGATCCCA
CGGGAGCCCACTCCAATGCACCAATCACAGCTCATGCCGAGGTGGAGGTA
GTGGAGGAAGCTAAGTGCTGCTGTTTCTTTAAGAGGAAAAGGAAGAAGAC
TGCTCAGCGCCACAAGTGAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDHPSREKDERQRTTKPMA
QRSAHCSRPSGSSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEY
VAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLE
LLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPEN
FLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMS
INTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDT
KRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKG
YTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQ
VVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQ
RHK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5583
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence difference compared with NM_001011664: t717c (silent in H239).; ; Estimated MW of expressed protein : 75333 Daltons