cDNA Clone EMAPL

• Plasmid: pEGFP-N1-EMAPL
• Parent Plasmid: pEGFPN1
• Expressed: EMAPL-GFP
• Genbank: AK093146
• Tag: GFP
• Species: Human

Sequence of Insert

GAATTCTGGCCACCATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGGGGTGG
CGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTG
TTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGAT
AAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGAC
GCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTG
GGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTG
GATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAAT
GAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCT
TCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTC
CACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCT
TACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCC
CTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAG
GGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGG
CAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTC
ATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTG
AGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGT
GGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCA
TTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCA
TTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCA
CACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCC
TCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCAT
GCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTT
CCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGT
TGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGC
AATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGC
CATTGGTTCCCATGACAACGTGATCTATATCTATAGTGTTTCCAGTGATG
GTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATC
ACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGG
GGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGA
AGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTG
CTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCC
GCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCAC
GACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCA
GTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCT
CTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTG
CCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCT
TCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGAC
TTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCC
CTGCACACACTGTTACGGTACC

Amino Acid Sequence

MELVKAALAEALRLLRLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPS
LVSRGTQTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSS
GAGSPGPPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSAN
LLVRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYI
PSGIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIAC
VVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVD
KDGKPLQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVV
DDSNEHMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHV
HFWNWSGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSE
GNILTWGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVL
SGGGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNA
LLRGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESH
ALAWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDG
NEQLSVVRYSPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFI
THLDWSKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCV
LGFHVYVPVRSCQGAEPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLP
VASAGRWGRGAGARHALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCP
SLAQSPTTRGRLFPGLTSRHSRSRIFLEGANGAPAHTVTVPPVATMVSKG
EELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLP
VPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA
DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ
SALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5571
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for mammalian expression of EMAPL with C-terminal GFP.; ; N.B. Sequence differences compared with AK093146 CDS:; a795g (silent G265); delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; c2028t (silent Y676),; ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388