cDNA Clone LMO7

• Plasmid: pCMV5-3HA LMO7 69-end
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA LMO7 69-end
• Genbank: NM_005358
• Tag: HA
• Species: Human

Sequence of Insert

ggtaccGGAAGGACTATTCTCATTAAGGCAGTAACAGAGAAGAATTTTGAAACAAAAGATTTTCGAGCCTCTCTAGAAAATGGTGTTCTGCTGTGTGATTTGATTAATAAGCTTAAACCTGGCGTCATTAAGAAGATCAATAGACTGTCTACACCAATAGCAGGATTGGATAATATAAACGTTTTCTTGAAAGCTTGTGAACAGATTGGATTGAAAGAAGCCCAGCTTTTCCATCCTGGAGATCTACAGGATTTATCAAATCGAGTCACTGTCAAGCAAGAAGAGACTGACAGGAGAGTGAAAAAT
GTTTTGATAACATTGTACTGGCTGGGAAGAAAAGCACAAAGCAACCCGTA
CTATAATGGTCCCCATCTTAATTTGAAAGCGTTTGAGAATCTTTTAGGAC
AAGCACTGACGAAGGCACTCGAAGACTCCAGCTTCCTGAAAAGAAGTGGC
AGGGACAGTGGCTACGGTGACATCTGGTGTCCTGAACGTGGAGAATTTCT
TGCTCCTCCAAGGCACCATAAGAGAGAAGATTCCTTTGAAAGCTTGGACT
CTTTGGGCTCGAGGTCATTGACAAGCTGCTCCTCTGATATCACGTTGAGA
GGGGGGCGTGAAGGTTTTGAAAGTGACACAGATTCGGAATTTACATTCAA
GATGCAGGATTATAATAAAGATGATATGTCGTATCGAAGGATTTCGGCTG
TTGAGCCAAAGACTGCGTTACCCTTCAATCGTTTTTTACCCAACAAAAGT
AGACAGCCATCCTATGTACCAGCACCTCTGAGAAAGAAAAAGCCAGACAA
ACATGAGGATAACAGAAGAAGTTGGGCAAGCCCGGTTTATACAGAAGCAG
ATGGAACATTTTCAAGGAGTAAGTCCATGAGTGATGTCAGCGCAGAAGAT
GTTCAAAACTTGCGTCAGCTGCGTTACGAGGAGATGCAGAAAATAAAATC
ACAATTAAAAGAACAAGATCAGAAATGGCAGGATGACCTTGCAAAATGGA
AAGATCGTCGAAAAAGTTACACTTCAGATCTGCAGAAGAAAAAAGAAGAG
AGAGAAGAAATTGAAAAGCAGGCACTTGAGAAGTCTAAGAGAAGCTCTAA
GACGTTTAAGGAAATGCTGCAGGACAGGGAATCCCAAAATCAAAAGTCTA
CAGTTCCGTCAAGAAGGAGAATGTATTCTTTTGATGATGTGCTGGAGGAA
GGAAAGCGACCCCCTACAATGACTGTGTCAGAAGCAAGTTACCAGAGTGA
GAGAGTAGAAGAGAAGGGAGCAACTTATCCTTCAGAAATTCCCAAAGAAG
ATTCTACCACTTTTGCAAAAAGAGAGGACCGTGTAACAACTGAAATTCAG
CTTCCTTCTCAAAGTCCTGTGGAAGAACAAAGCCCAGCCTCTTTGTCTTC
TCTGCGTTCACGGAGCACACAAATGGAATCAACTTGTGTTTCAGCTTCTC
TCCCCAGAAGTTACCGGAAAACTGATACAGTCAGGTTAACATCTGTGGTC
ACACCAAGACCCTTTGGCTCTCAGACAAGGGGAATCTCATCACTCCCCAG
ATCTTACACGATGGATGATGCTTGGAAGTATAATGGAGATATTGAAGACA
TTAAGAGAACTCCAAACAATGTGGTCAGCACCCCTGCACCAAGCCCGGAC
GCAAGCCAACTGGCTTCAAGCTTATCTAGCCAGAAAGAGGTAGCAGCAAC
AGAAGAAGATGTGACAAGGCTGCCCTCTCCTACATCCCCCTTCTCATCTC
TTTCCCAAGACCAGGCTGCCACTTCTAAAGCCACATTGTCTTCCACATCT
GGTCTTGATTTAATGTCTGAATCTGGAGAAGGGGAAATCTCCCCACAAAG
AGAAGTCTCAAGATCCCAGGATCAGTTCAGTGATATGAGAATCAGCATAA
ACCAGACGCCTGGGAAGAGTCTTGACTTTGGGTTTACAATAAAATGGGAT
ATTCCTGGGATCTTCGTAGCATCAGTTGAAGCAGGTAGCCCAGCAGAATT
TTCTCAGCTACAAGTAGATGATGAAATTATTGCTATTAACAACACCAAGT
TTTCATATAACGATTCAAAAGAGTGGGAGGAAGCCATGGCTAAGGCTCAA
GAAACTGGACACCTAGTGATGGATGTGAGGCGCTATGGAAAGGCTGGTTC
ACCTGAAACAAAGTGGATTGATGCAACTTCTGGAATTTACAACTCAGAAA
AATCTTCAAATCTATCTGTAACAACTGATTTCTCCGAAAGCCTTCAGAGT
TCTAATATTGAATCCAAAGAAATCAATGGAATTCATGATGAAAGCAATGC
TTTTGAATCAAAAGCATCTGAATCCATTTCTTTGAAAAACTTAAAAAGGC
GATCACAATTTTTTGAACAAGGAAGCTCTGATTCGGTGGTTCCTGATCTT
CCAGTTCCAACCATCAGTGCCCCGAGTCGCTGGGTGTGGGATCAAGAGGA
GGAGCGGAAGCGGCAGGAGAGGTGGCAGAAGGAGCAGGACCGCCTACTGC
AGGAAAAATATCAACGTGAGCAGGAGAAACTGAGGGAAGAGTGGCAAAGG
GCCAAACAGGAGGCAGAGAGAGAGAATTCCAAGTACTTGGATGAGGAACT
GATGGTCCTAAGCTCAAACAGCATGTCTCTGACCACACGGGAGCCCTCTC
TTGCCACCTGGGAAGCTACCTGGAGTGAAGGGTCCAAGTCTTCAGACAGA
GAAGGAACCCGAGCAGGAGAAGAGGAGAGGAGACAGCCACAAGAGGAAGT
TGTTCATGAGGACCAAGGAAAGAAGCCGCAGGATCAGCTTGTTATTGAGA
GAGAGAGGAAATGGGAGCAACAGCTTCAGGAAGAGCAAGAGCAAAAGCGG
CTTCAGGCTGAGGCTGAGGAGCAGAAGCGTCCTGCGGAGGAGCAGAAGCG
CCAGGCAGAGATAGAGCGGGAAACATCAGTCAGAATATACCAGTACAGGA
GGCCTGTTGATTCCTATGATATACCAAAGACAGAAGAAGCATCTTCAGGT
TTTCTTCCTGGTGACAGGAATAAATCCAGATCTACTACTGAACTGGATGA
TTACTCCACAAATAAAAATGGAAACAATAAATATTTAGACCAAATTGGGA
ACACGACCTCTTCACAGAGGAGATCCAAGAAAGAACAAGTACCATCAGGA
GCAGAATTGGAGAGGCAACAAATCCTTCAGGAAATGAGGAAGAGAACACC
CCTTCACAATGACAACAGCTGGATCCGACAGCGCAGTGCCAGTGTCAACA
AAGAGCCTGTTAGTCTTCCTGGGATCATGAGAAGAGGCGAATCTTTAGAT
AACCTGGACTCCCCCCGATCCAATTCTTGGAGACAGCCTCCTTGGCTCAA
TCAGCCCACAGGATTCTATGCTTCTTCCTCTGTGCAAGACTTTAGTCGCC
CACCACCTCAGCTGGTGTCCACATCAAACCGTGCCTACATGCGGAACCCC
TCCTCCAGCGTGCCCCCACCTTCAGCTGGCTCCGTGAAGACCTCCACCAC
AGGTGTGGCCACCACACAGTCCCCCACCCCGAGAAGCCATTCCCCTTCAG
CTTCACAGTCAGGCTCTCAGCTGCGTAACAGGTCAGTCAGTGGGAAGCGC
ATATGCTCCTACTGCAATAACATTCTGGGCAAAGGAGCCGCCATGATCAT
CGAGTCCCTGGGTCTTTGTTATCATTTGCATTGTTTTAAGTGTGTTGCCT
GTGAGTGTGACCTCGGAGGCTCTTCCTCAGGAGCTGAAGTCAGGATCAGA
AACCACCAACTGTACTGCAACGACTGCTATCTCAGATTCAAATCTGGACG
GCCAACCGCCATGTGAgtcgac

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFRSGTGRTILIKAVTEKNF
ETKDFRASLENGVLLCDLINKLKPGVIKKINRLSTPIAGLDNINVFLKAC
EQIGLKEAQLFHPGDLQDLSNRVTVKQEETDRRVKNVLITLYWLGRKAQS
NPYYNGPHLNLKAFENLLGQALTKALEDSSFLKRSGRDSGYGDIWCPERG
EFLAPPRHHKREDSFESLDSLGSRSLTSCSSDITLRGGREGFESDTDSEF
TFKMQDYNKDDMSYRRISAVEPKTALPFNRFLPNKSRQPSYVPAPLRKKK
PDKHEDNRRSWASPVYTEADGTFSRSKSMSDVSAEDVQNLRQLRYEEMQK
IKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKR
SSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASY
QSERVEEKGATYPSEIPKEDSTTFAKREDRVTTEIQLPSQSPVEEQSPAS
LSSLRSRSTQMESTCVSASLPRSYRKTDTVRLTSVVTPRPFGSQTRGISS
LPRSYTMDDAWKYNGDIEDIKRTPNNVVSTPAPSPDASQLASSLSSQKEV
AATEEDVTRLPSPTSPFSSLSQDQAATSKATLSSTSGLDLMSESGEGEIS
PQREVSRSQDQFSDMRISINQTPGKSLDFGFTIKWDIPGIFVASVEAGSP
AEFSQLQVDDEIIAINNTKFSYNDSKEWEEAMAKAQETGHLVMDVRRYGK
AGSPETKWIDATSGIYNSEKSSNLSVTTDFSESLQSSNIESKEINGIHDE
SNAFESKASESISLKNLKRRSQFFEQGSSDSVVPDLPVPTISAPSRWVWD
QEEERKRQERWQKEQDRLLQEKYQREQEKLREEWQRAKQEAERENSKYLD
EELMVLSSNSMSLTTREPSLATWEATWSEGSKSSDREGTRAGEEERRQPQ
EEVVHEDQGKKPQDQLVIERERKWEQQLQEEQEQKRLQAEAEEQKRPAEE
QKRQAEIERETSVRIYQYRRPVDSYDIPKTEEASSGFLPGDRNKSRSTTE
LDDYSTNKNGNNKYLDQIGNTTSSQRRSKKEQVPSGAELERQQILQEMRK
RTPLHNDNSWIRQRSASVNKEPVSLPGIMRRGESLDNLDSPRSNSWRQPP
WLNQPTGFYASSSVQDFSRPPPQLVSTSNRAYMRNPSSSVPPPSAGSVKT
STTGVATTQSPTPRSHSPSASQSGSQLRNRSVSGKRICSYCNNILGKGAA
MIIESLGLCYHLHCFKCVACECDLGGSSSGAEVRIRNHQLYCNDCYLRFK
SGRPTAM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5563
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with NM_005358 CDS:; g1825a (V609I) - in several ests, other mRNA seqs, and rat and mouse seqs. delta nt1070-1099 (aaL358-G367) – in several ESTs; a2304g (silent K768) c852t (silent F284); ; Estimated MW of expressed protein : 148458 Daltons