cDNA Clone PTIP

• Plasmid: pET28a h.PTIP G316-L591
• Parent Plasmid: pET28a original
• Expressed: 6His-PTIP G316-L591
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccGGACAAAACCTCCAAAGTTCTGAAAGATCAGAAATGATAGCTACCTGGAGTCCAGCTGTACGGACACTGAGGAATATTACTAATAATGCTGACATTCAGCAGATGAACCGGCCATCAAATGTAGCACATATCCTACAGACTCTCTCAGCACCTACGAAAAATTTAGAACAGCAGGTGAATCACAGCCAGCAGGGACATACAAATGCCAATGCAGTGCTGTTTAGCCAAGTGAAAGTGACTCCAGAGACACACATGCTACAGCAGCAGCAGCAGGCCCAGCAGCAGCAGCAGCAGCACCCG
GTTTTACACCTTCAGCCCCAGCAGATAATGCAGCTCCAGCAGCAGCAGCA
GCAGCAGATCTCTCAGCAACCTTACCCCCAGCAGCCGCCGCATCCATTTT
CACAGCAACAGCAGCAGCAGCAGCAAGCCCATCCGCATCAGTTTTCACAG
CAACAGCTACAGTTTCCACAGCAACAGTTGCATCCTCCACAGCAGCTGCA
TCGCCCTCAGCAGCAGCTCCAGCCCTTTCAGCAGCAGCATGCCCTGCAGC
AGCAGTTCCATCAGCTGCAGCAGCACCAGCTCCAGCAGCAGCAGCTCGCC
CAGCTCCAGCAGCAGCACAGCCTGCTCCAGCAGCAGCAGCAACAGCAGAT
TCAGCAGCAGCAGCTCCAGCGCATGCACCAGCAGCAGCAGCAGCAGCAGA
TGCAAAGTCAGACAGCGCCACACTTGAGTCAGACGTCACAGGCGCTGCAG
CATCAGGTTCCACCTCAGCAGCCCCCGCAGCAGCAGCAGCAACAGCAGCC
ACCACCATCGCCTCAGCAGCATCAGCTTTGAggatcc

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSGQNLQSSERSEMIATW
SPAVRTLRNITNNADIQQMNRPSNVAHILQTLSAPTKNLEQQVNHSQQGH
TNANAVLFSQVKVTPETHMLQQQQQAQQQQQQHPVLHLQPQQIMQLQQQQ
QQQISQQPYPQQPPHPFSQQQQQQQQAHPHQFSQQQLQFPQQQLHPPQQL
HRPQQQLQPFQQQHALQQQFHQLQQHQLQQQQLAQLQQQHSLLQQQQQQQ
IQQQQLQRMHQQQQQQQMQSQTAPHLSQTSQALQHQVPPQQPPQQQQQQQ
PPPSPQQHQL*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU5553
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for expression of hPTIP 316-591 with N-terminal 6His and T7 tags.