cDNA Clone MEF2a

• Plasmid: pGEX6P-1-Mef2a
• Parent Plasmid: pGEX6P1
• Expressed: GST-Mef2a
• Genbank: NM_005587*
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGGGCGGAAGAAAATACAAATCACACGCATAATGGATGAAAGGAACCGACAGGTCACTTTTACAAAGAGAAAGTTTGGATTAATGAAGAAAGCCTATGAACTTAGTGTGCTCTGTGACTGTGAAATAGCACTCATCATTTTCAACAGCTCTAACAAACTGTTTCAATATGCTAGCACTGATATGGACAAAGTTCTTCTCAAGTATACAGAATATAATGAACCTCATGAAAGCAGAACCAACTCGGATATTGTTGAGGCTCTGAACAAGAAGGAACACAGAGGGTGCGACAGCCCAGAC
CCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA
AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTG
GTCTGCCACCTCAGAACTTTTCAATGTCTGTCACAGTTCCAGTGACCAGC
CCCAATGCTTTGTCCTACACTAACCCAGGGAGTTCACTGGTGTCCCCATC
TTTGGCAGCCAGCTCAACGTTAACAGATTCAAGCATGCTCTCTCCACCTC
AAACCACATTACATAGAAATGTGTCTCCTGGAGCTCCTCAGAGACCACCA
AGTACTGGCAATGCAGGTGGGATGTTGAGCACTACAGACCTCACAGTGCC
AAATGGAGCTGGAAGCAGTCCAGTGGGGAATGGATTTGTAAACTCAAGAG
CTTCTCCAAATTTGATTGGAGCTACTGGTGCAAATAGCTTAGGCAAAGTC
ATGCCTACAAAGTCTCCCCCTCCACCAGGTGGTGGTAATCTTGGAATGAA
CAGTAGGAAACCAGATCTTCGAGTTGTCATCCCCCCTTCAAGCAAGGGCA
TGATGCCTCCACTAAATACCCAAAGGATCAGTAGTTCTCAAGCCACTCAA
CCTCTTGCTACCCCAGTCGTGTCTGTGACAACCCCAAGCTTGCCTCCGCA
AGGACTTGTGTACTCAGCAATGCCGACTGCCTACAACACTGATTATTCAC
TGACCAGCGCTGACCTGTCAGCCCTTCAAGGCTTCAACTCGCCAGGAATG
CTGTCGCTGGGACAGGTGTCGGCCTGGCAGCAGCACCACCTAGGACAAGC
AGCCCTCAGCTCTCTTGTTGCTGGAGGGCAGTTATCTCAGGGTTCCAATT
TATCCATTAATACCAACCAAAACATCAGCATCAAGTCCGAACCGATTTCA
CCTCCTCGGGATCGTATGACCCCATCGGGCTTCCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCCGCCGCCACCACCGCAGCCCCAGCCACAACCCC
CGCAGCCCCAGCCCCGACAGGAAATGGGGCGCTCCCCTGTGGACAGTCTG
AGCAGCTCTAGTAGCTCCTATGATGGCAGTGATCGGGAGGATCCACGGGG
CGACTTCCATTCTCCAATTGTGCTTGGCCGACCCCCAAACACTGAGGACA
GAGAAAGCCCTTCTGTAAAGCGAATGAGGATGGACGCGTGGGTGACCTAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMGRKKIQITRIMDERN
RQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVL
LKYTEYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYK
KINEEFDNMMRNHKIAPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSP
SLAASSTLTDSSMLSPPQTTLHRNVSPGAPQRPPSTGNAGGMLSTTDLTV
PNGAGSSPVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGM
NSRKPDLRVVIPPSSKGMMPPLNTQRISSSQATQPLATPVVSVTTPSLPP
QGLVYSAMPTAYNTDYSLTSADLSALQGFNSPGMLSLGQVSAWQQHHLGQ
AALSSLVAGGQLSQGSNLSINTNQNISIKSEPISPPRDRMTPSGFQQQQQ
QQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPR
GDFHSPIVLGRPPNTEDRESPSVKRMRMDAWVT*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5543
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; *N.B. Difference in cloned sequence compared with NM_005587 CDS:; Deletion of nucleotides 865-888 (residues S289-L296) – Same region deleted in majority of EST sequences.; c891t (silent N297); ; ; Estimated MW of expressed protein : 80998 Daltons