cDNA Clone MEF2a

• Plasmid: pEBG6P-1 MEF2a
• Parent Plasmid: pEBG6P
• Expressed: GST-MEF2a
• Genbank: NM_005587*
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGGGGCGGAAGAAAATACAAATCACACGCATAATGGATGAAAGGAACCGACAGGTCACTTTTACAAAGAGAAAGTTTGGATTAATGAAGAAAGCCTATGAACTTAGTGTGCTCTGTGACTGTGAAATAGCACTCATCATTTTCAACAGCTCTAACAAACTGTTTCAATATGCTAGCACTGATATGGACAAAGTTCTTCTCAAGTATACAGAATATAATGAACCTCATGAAAGCAGAACCAACTCGGATATTGTTGAGGCTCTGAACAAGAAGGAACACAGAGGGTGCGACAGCCCAGAC
CCTGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAA
AATTAATGAGGAATTTGATAATATGATGCGGAATCATAAAATCGCACCTG
GTCTGCCACCTCAGAACTTTTCAATGTCTGTCACAGTTCCAGTGACCAGC
CCCAATGCTTTGTCCTACACTAACCCAGGGAGTTCACTGGTGTCCCCATC
TTTGGCAGCCAGCTCAACGTTAACAGATTCAAGCATGCTCTCTCCACCTC
AAACCACATTACATAGAAATGTGTCTCCTGGAGCTCCTCAGAGACCACCA
AGTACTGGCAATGCAGGTGGGATGTTGAGCACTACAGACCTCACAGTGCC
AAATGGAGCTGGAAGCAGTCCAGTGGGGAATGGATTTGTAAACTCAAGAG
CTTCTCCAAATTTGATTGGAGCTACTGGTGCAAATAGCTTAGGCAAAGTC
ATGCCTACAAAGTCTCCCCCTCCACCAGGTGGTGGTAATCTTGGAATGAA
CAGTAGGAAACCAGATCTTCGAGTTGTCATCCCCCCTTCAAGCAAGGGCA
TGATGCCTCCACTAAATACCCAAAGGATCAGTAGTTCTCAAGCCACTCAA
CCTCTTGCTACCCCAGTCGTGTCTGTGACAACCCCAAGCTTGCCTCCGCA
AGGACTTGTGTACTCAGCAATGCCGACTGCCTACAACACTGATTATTCAC
TGACCAGCGCTGACCTGTCAGCCCTTCAAGGCTTCAACTCGCCAGGAATG
CTGTCGCTGGGACAGGTGTCGGCCTGGCAGCAGCACCACCTAGGACAAGC
AGCCCTCAGCTCTCTTGTTGCTGGAGGGCAGTTATCTCAGGGTTCCAATT
TATCCATTAATACCAACCAAAACATCAGCATCAAGTCCGAACCGATTTCA
CCTCCTCGGGATCGTATGACCCCATCGGGCTTCCAGCAGCAGCAGCAGCA
GCAGCAGCAGCAGCAGCCGCCGCCACCACCGCAGCCCCAGCCACAACCCC
CGCAGCCCCAGCCCCGACAGGAAATGGGGCGCTCCCCTGTGGACAGTCTG
AGCAGCTCTAGTAGCTCCTATGATGGCAGTGATCGGGAGGATCCACGGGG
CGACTTCCATTCTCCAATTGTGCTTGGCCGACCCCCAAACACTGAGGACA
GAGAAAGCCCTTCTGTAAAGCGAATGAGGATGGACGCGTGGGTGACCTAAactagt

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMGRKKIQITRI
MDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTD
MDKVLLKYTEYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHT
EEKYKKINEEFDNMMRNHKIAPGLPPQNFSMSVTVPVTSPNALSYTNPGS
SLVSPSLAASSTLTDSSMLSPPQTTLHRNVSPGAPQRPPSTGNAGGMLST
TDLTVPNGAGSSPVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGG
GNLGMNSRKPDLRVVIPPSSKGMMPPLNTQRISSSQATQPLATPVVSVTT
PSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFNSPGMLSLGQVSAWQQ
HHLGQAALSSLVAGGQLSQGSNLSINTNQNISIKSEPISPPRDRMTPSGF
QQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSD
REDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDAWVT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5542
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express MEF2A with N-terminal GST (contains preScission protease site); *N.B. Difference in cloned sequence compared with NM_005587 CDS:; Deletion of nucleotides 865-888 (residues S289-L296) – Same region deleted in majority of EST sequences.; c891t (silent N297); ; Estimated MW of expressed protein : 53818 Daltons