cDNA Clone HIPK3

• Plasmid: pFastBac.HTa HIPK3 161-562
• Parent Plasmid: pSC-A
• Expressed: 6His-HIPK3 161-562
• Genbank: NM_005734
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcCCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGA
GCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGAT
GCTGGAACAAAACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCC
TGCCACTAAAAGTGATTCGGCCCATTCTTCAACAAGTGGCCACTGCACTG
AAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA
TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAG
ACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTA
CAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGTTGCCATTTTG
TGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTC
TTGGATGGCCGCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATAC
ATTTCTCAGACTCAAGGTTTGCCAGGAGAACAGTTGTTAAATGTGGGTAC
TAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT
GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCT
AAAGAAGCCAGAAAATACATTTTCAACAGTCTGGATGATGTAGCGCATGT
GAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCTGAGAAAGCTG
ATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCA
GATTTAAGAATTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATAT
GAAACATCTTCTAGATTTCCCTCATAGCAACCATGTAAAGTCCTGTTTTC
ATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT
CACAACTGAgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFPVTVVTATTGSKQNCTTGEG
DYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKN
HPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQ
NLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIML
VDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAI
DMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKST
RFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTV
MDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHL
LDFPHSNHVKSCFHIMDICKSHLNSCDTNNHN*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5533
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 49516 Daltons