cDNA Clone SIRT6

• Plasmid: pET28a 6His.SIRT6
• Parent Plasmid: pET28a original
• Expressed: 6His-SIRT6
• Genbank: AAH05026
• Tag: 6His
• Species: Human

Sequence of Insert

gctagcATGTCGGTGAATTACGCGGCGGGGCTGTCGCCGTACGCGGACAAGGGCAAGTGCGGCCTCCCGGAGATCTTCGACCCCCCGGAGGAGCTGGAGCGGAAGGTGTGGGAACTGGCGAGGCTGGTCTGGCAGTCTTCCAGTGTGGTGTTCCACACGGGTGCCGGCATCAGCACTGCCTCTGGCATCCCCGACTTCAGGGGTCCCCACGGAGTCTGGACCATGGAGGAGCGAGGTCTGGCCCCCAAGTTCGACACCACCTTTGAGAGCGCGCGGCCCACGCAGACCCACATGGCGCTGGTGCAG
CTGGAGCGCGTGGGCCTCCTCCGCTTCCTGGTCAGCCAGAACGTGGACGG
GCTCCATGTGCGCTCAGGCTTCCCCAGGGACAAACTGGCAGAGCTCCACG
GGAACATGTTTGTGGAAGAATGTGCCAAGTGTAAGACGCAGTACGTCCGA
GACACAGTCGTGGGCACCATGGGCCTGAAGGCCACGGGCCGGCTCTGCAC
CGTGGCTAAGGCAAGGGGGCTGCGAGCCTGCAGGGGAGAGCTGAGGGACA
CCATCCTAGACTGGGAGGACTCCCTGCCCGACCGGGACCTGGCACTCGCC
GATGAGGCCAGCAGGAACGCCGACCTGTCCATCACGCTGGGTACATCGCT
GCAGATCCGGCCCAGCGGGAACCTGCCGCTGGCTACCAAGCGCCGGGGAG
GCCGCCTGGTCATCGTCAACCTGCAGCCCACCAAGCACGACCGCCATGCT
GACCTCCGCATCCATGGCTACGTTGACGAGGTCATGACCCGGCTCATGAA
GCACCTGGGGCTGGAGATCCCCGCCTGGGACGGCCCCCGTGTGCTGGAGA
GGGCGCTGCCACCCCTGCCCCGCCCGCCCACCCCCAAGCTGGAGCCCAAG
GAGGAATCTCCCACCCGGATCAACGGCTCTATCCCCGCCGGCCCCAAGCA
GGAGCCCTGCGCCCAGCACAACGGCTCAGAGCCCGCCAGCCCCAAACGGG
AGCGGCCCACCAGCCCTGCCCCCCACAGACCCCCCAAAAGGGTGAAGGCC
AAGGCGGTCCCCAGCTGAggatcc

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMSVNYAAGLSPYADKGKCGLPEIFDPP
EELERKVWELARLVWQSSSVVFHTGAGISTASGIPDFRGPHGVWTMEERG
LAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRD
KLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRAC
RGELRDTILDWEDSLPDRDLALADEASRNADLSITLGTSLQIRPSGNLPL
ATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWD
GPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSE
PASPKRERPTSPAPHRPPKRVKAKAVPS*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU5527
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for expression of human SIRT6 with N-terminal 6His tag.