cDNA Clone PTIP

• Plasmid: pET28a 6His.T7.h.PTIP 841-1069
• Parent Plasmid: pET28a original
• Expressed: 6His-T7-PTIP 841-1069
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccAATGTCCAGCCTTCTTCCAAAAGAGCCAGAATTGAAGACGTACCACCTCCCACTAAAAAGCTAACTCCAGAATTGACCCCTTTTGTGCTTTTCACTGGATTCGAGCCTGTCCAGGTTCAACAGTATATTAAGAAGCTCTACATTCTTGGTGGAGAGGTTGCGGAGTCTGCACAGAAGTGCACACACCTCATTGCCAGCAAGGTGACTCGCACCGTGAAGTTCCTGACGGCGATTTCTGTCGTGAAGCACATAGTGACGCCAGAGTGGCTGGAAGAATGCTTCAGGTGTCAGAAGTTCATT
GATGAGCAGAACTACATTCTCCGAGATGCTGAGGCAGAAGTACTTTTCTC
TTTCAGCTTGGAAGAATCCTTAAAACGGGCACACGTTTCTCCACTCTTTA
AGGCAAAATATTTTTACATCACACCTGGAATCTGCCCAAGTCTTTCCACT
ATGAAGGCAATCGTAGAGTGTGCAGGAGGAAAGGTGTTATCCAAGCAGCC
ATCTTTCCGGAAGCTCATGGAGCACAAGCAGAACTCGAGTTTGTCGGAAA
TAATTTTAATATCCTGTGAAAATGACCTTCATTTATGCCGAGAATATTTT
GCCAGAGGCATAGATGTTCACAATGCAGAGTTCGTTCTGACTGGAGTGCT
CACTCAAACGCTGGACTATGAATCATATAAGTTTAACTGAggatcc

Amino Acid Sequence

MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSNVQPSSKRARIEDVPP
PTKKLTPELTPFVLFTGFEPVQVQQYIKKLYILGGEVAESAQKCTHLIAS
KVTRTVKFLTAISVVKHIVTPEWLEECFRCQKFIDEQNYILRDAEAEVLF
SFSLEESLKRAHVSPLFKAKYFYITPGICPSLSTMKAIVECAGGKVLSKQ
PSFRKLMEHKQNSSLSEIILISCENDLHLCREYFARGIDVHNAEFVLTGV
LTQTLDYESYKFN*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU5526
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for expression of hPTIP 841-1069(end) with N-terminal 6His and T7 tags.