cDNA Clone HIPK3

• Plasmid: pGEX6P-1-HIPK3 161-562
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIPK3 161-562
• Genbank: NM_005734.3 GI:114796623
• Tag: GST
• Species: Human

Sequence of Insert

gaattcCCAGTGACAGTTGTGACAGCTACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGCTGATGAATATAACTTTGTACGA
GCTTATGAATGCTTTCAGCACCGTAACCATACTTGTTTAGTCTTTGAGAT
GCTGGAACAAAACTTGTATGACTTTCTGAAACAAAATAAATTTAGTCCCC
TGCCACTAAAAGTGATTCGGCCCATTCTTCAACAAGTGGCCACTGCACTG
AAAAAATTGAAAAGTCTTGGTTTAATTCATGCTGATCTCAAGCCAGAGAA
TATTATGTTGGTGGATCCTGTTCGGCAGCCTTACAGGGTTAAAGTAATAG
ACTTTGGGTCGGCCAGTCATGTATCAAAGACTGTTTGTTCAACATATCTA
CAATCTCGGTACTACAGAGCTCCAGAGATTATATTGGGGTTGCCATTTTG
TGAAGCCATAGACATGTGGTCATTGGGATGTGTGATTGCAGAATTATTTC
TTGGATGGCCGCTCTACCCAGGAGCCTTGGAGTATGATCAGATTCGATAC
ATTTCTCAGACTCAAGGTTTGCCAGGAGAACAGTTGTTAAATGTGGGTAC
TAAATCCACAAGATTTTTTTGCAAAGAAACAGATATGTCTCATTCTGGTT
GGAGATTAAAGACATTGGAAGAGCATGAGGCAGAGACAGGAATGAAGTCT
AAAGAAGCCAGAAAATACATTTTCAACAGTCTGGATGATGTAGCGCATGT
GAACACAGTGATGGATTTGGAAGGAAGTGATCTTTTGGCTGAGAAAGCTG
ATAGAAGAGAATTTGTTAGTCTGTTGAAGAAAATGTTGCTGATTGATGCA
GATTTAAGAATTACTCCAGCTGAGACCCTGAACCATCCTTTTGTTAATAT
GAAACATCTTCTAGATTTCCCTCATAGCAACCATGTAAAGTCCTGTTTTC
ATATTATGGATATTTGTAAGTCCCACCTAAATTCATGTGACACAAATAAT
CACAACTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFPVTVVTATTGSKQNCT
TGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIK
ILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFE
MLEQNLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPE
NIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPF
CEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVG
TKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAH
VNTVMDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVN
MKHLLDFPHSNHVKSCFHIMDICKSHLNSCDTNNHN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5525
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 72983 Daltons