cDNA Clone HIPK2

• Plasmid: pGEX6P-1-HIPK2 165-564
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIPK2 165-564
• Genbank: AF326592
• Tag: GST
• Species: Human

Sequence of Insert

gaattcACTGCCACCACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACTATCAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAGGTCTTAGAGTTCTTGGGCCGAGGGACGTTTGGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAGATCGTGGCCATCAAGATCCTGAAGAACCACCCATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTGAGCACGGAGAGTGCCGATGACTATAACTTCGTCCGGGCCTAC
GAATGCTTCCAGCACAAGAACCACACGTGCTTGGTCTTCGAGATGTTGGA
GCAGAACCTCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCC
TCAAATACATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAA
CTCAAAAGCCTAGGTCTTATCCACGCTGACCTCAAACCAGAAAACATCAT
GCTGGTGGATCCATCTAGACAACCATACAGAGTCAAGGTCATCGACTTTG
GTTCAGCCAGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCC
AGATATTACAGGGCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGC
AATTGACATGTGGTCCCTGGGCTGTGTTATTGCAGAATTGTTCCTGGGTT
GGCCGTTATATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCA
CAAACACAGGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGAC
AACTAGGTTTTTCAACCGTGACACGGACTCACCATATCCTTTGTGGAGAC
TGAAGACACCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAA
GCAAGAAAGTACATTTTCATCTGTTTAGATGATATGGCCCAGGTGAACAT
GACGACAGATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGC
GGGAGTTCATTGACCTGTTGAAGAAGATGCTGACCATTGATGCTGACAAG
AGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACA
CTTACTCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACA
TGGAGATCTGCAAGCGTCGGGTGAATATGTATGACACGGTGAACCAGAGC
TAAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFTATTSTATSKNSGSNS
EGDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKIL
KNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEML
EQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENI
MLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCE
AIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTK
TTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFICLDDMAQVN
MTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMT
HLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 72917 Daltons