cDNA Clone HIPK1

• Plasmid: pGEX6P-1-HIPK1 158-555
• Parent Plasmid: pGEX6P1
• Expressed: GST-HIPK1 158-555
• Genbank: NM_198268
• Tag: GST
• Species: Human

Sequence of Insert

gaattcACAACCACCACTGTGACCACAAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGACCAATAGCTATGAAGTCTTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCTAAGTGCTGGAAGAGGAGCACCAAGGAAATTGTGGCTATTAAAATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTGAAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAATTTTGTCCGTTCATACGAGTGC
TTTCAGCATAAGAATCACACCTGCCTTGTTTTTGAAATGTTGGAGCAGAA
CTTATATGATTTTCTAAAGCAAAACAAATTTAGCCCACTGCCACTCAAGT
ACATCAGACCAATCTTGCAGCAGGTGGCCACAGCCTTGATGAAGCTCAAG
AGTCTTGGTCTGATCCACGCTGACCTTAAGCCTGAAAACATCATGCTGGT
TGATCCAGTTCGCCAGCCCTACCGAGTGAAGGTCATTGACTTTGGTTCTG
CTAGTCACGTTTCCAAAGCTGTGTGCTCAACCTACTTACAGTCACGTTAC
TACAGAGCTCCTGAAATTATTCTTGGGTTACCATTTTGTGAAGCTATTGA
TATGTGGTCACTGGGCTGTGTGATAGCTGAGCTGTTCCTGGGATGGCCTC
TTTATCCTGGTGCTTCAGAATATGATCAGATTCGTTATATTTCACAAACA
CAAGGCTTGCCAGCTGAATATCTTCTCAGTGCCGGAACAAAAACAACCAG
GTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGA
CACCTGAAGAACATGAACTGGAGACTGGAATAAAATCAAAAGAAGCTCGG
AAGTACATTTTTAATTGCTTAGATGACATGGCTCAGGTGAATATGTCTAC
AGACCTGGAGGGAACAGACATGTTGGCAGAGAAGGCAGACCGAAGAGAAT
ACATTGATCTGTTAAAGAAAATGCTCACAATTGATGCAGATAAGAGAATT
ACCCCTCTAAAAACTCTTAACCATCAGTTTGTGACAATGACTCACCTTTT
GGATTTTCCACATAGCAATCATGTTAAGTCTTGTTTTCAGAACATGGAGA
TCTGCAAGCGGAGGGTTCACATGTATGATACAGTGAGTCAGATCTAGgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFTTTTVTTKSSSSSGEG
DYQLVQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKN
HPSYARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQ
NLYDFLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIML
VDPVRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAI
DMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTT
RFFNRDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMS
TDLEGTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHL
LDFPHSNHVKSCFQNMEICKRRVHMYDTVSQI*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5523
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 72954 Daltons