cDNA Clone HIPK1

• Plasmid: pFastBac.HTa HIPK1 158-555
• Parent Plasmid: pSC-A
• Expressed: 6His-HIPK1 158-555
• Genbank: NM_198268
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcACAACCACCACTGTGACCACAAAGAGTAGCAGTTCCAGCGGAGAAGGGGATTACCAGCTGGTCCAGCATGAGATCCTTTGCTCTATGACCAATAGCTATGAAGTCTTGGAGTTCCTAGGCCGGGGGACATTTGGACAGGTGGCTAAGTGCTGGAAGAGGAGCACCAAGGAAATTGTGGCTATTAAAATCTTGAAGAACCACCCCTCCTATGCCAGACAAGGACAGATTGAAGTGAGCATCCTTTCCCGCCTAAGCAGTGAAAATGCTGATGAGTATAATTTTGTCCGTTCATACGAGTGC
TTTCAGCATAAGAATCACACCTGCCTTGTTTTTGAAATGTTGGAGCAGAA
CTTATATGATTTTCTAAAGCAAAACAAATTTAGCCCACTGCCACTCAAGT
ACATCAGACCAATCTTGCAGCAGGTGGCCACAGCCTTGATGAAGCTCAAG
AGTCTTGGTCTGATCCACGCTGACCTTAAGCCTGAAAACATCATGCTGGT
TGATCCAGTTCGCCAGCCCTACCGAGTGAAGGTCATTGACTTTGGTTCTG
CTAGTCACGTTTCCAAAGCTGTGTGCTCAACCTACTTACAGTCACGTTAC
TACAGAGCTCCTGAAATTATTCTTGGGTTACCATTTTGTGAAGCTATTGA
TATGTGGTCACTGGGCTGTGTGATAGCTGAGCTGTTCCTGGGATGGCCTC
TTTATCCTGGTGCTTCAGAATATGATCAGATTCGTTATATTTCACAAACA
CAAGGCTTGCCAGCTGAATATCTTCTCAGTGCCGGAACAAAAACAACCAG
GTTTTTCAACAGAGATCCTAATTTGGGGTACCCACTGTGGAGGCTTAAGA
CACCTGAAGAACATGAACTGGAGACTGGAATAAAATCAAAAGAAGCTCGG
AAGTACATTTTTAATTGCTTAGATGACATGGCTCAGGTGAATATGTCTAC
AGACCTGGAGGGAACAGACATGTTGGCAGAGAAGGCAGACCGAAGAGAAT
ACATTGATCTGTTAAAGAAAATGCTCACAATTGATGCAGATAAGAGAATT
ACCCCTCTAAAAACTCTTAACCATCAGTTTGTGACAATGACTCACCTTTT
GGATTTTCCACATAGCAATCATGTTAAGTCTTGTTTTCAGAACATGGAGA
TCTGCAAGCGGAGGGTTCACATGTATGATACAGTGAGTCAGATCTAGgaattc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFTTTTVTTKSSSSSGEGDYQL
VQHEILCSMTNSYEVLEFLGRGTFGQVAKCWKRSTKEIVAIKILKNHPSY
ARQGQIEVSILSRLSSENADEYNFVRSYECFQHKNHTCLVFEMLEQNLYD
FLKQNKFSPLPLKYIRPILQQVATALMKLKSLGLIHADLKPENIMLVDPV
RQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWS
LGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFN
RDPNLGYPLWRLKTPEEHELETGIKSKEARKYIFNCLDDMAQVNMSTDLE
GTDMLAEKADRREYIDLLKKMLTIDADKRITPLKTLNHQFVTMTHLLDFP
HSNHVKSCFQNMEICKRRVHMYDTVSQI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5522
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; ; Estimated MW of expressed protein : 49487 Daltons