cDNA Clone E1a

• Plasmid: pGFP-TAP-BN E1a
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP E1a
• Genbank: P22314/X56976
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

ggatccTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGACATA
GACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAAT
GAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGG
GCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACC
CTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTA
CCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCC
GCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGACCC
CTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAACAC
CCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGGCA
TCAAGCTGGTGGTGGCAGGCACGCGGGGCCTGTTTGGGCAGCTCTTCTGT
GACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACT
CAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCT
GCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCCTTT
TCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGAGAT
CAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACTTCT
CCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAG
ATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGT
GACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCTTCC
AGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCCCGC
AATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAATGC
TCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCATCC
GGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCCTTC
ATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTT
CATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTC
AGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCGCCAGAACCGT
TATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCTGGG
CAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGCTGC
TCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAAATC
ATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACAGTT
TCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTG
CAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAGAAC
CGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCAAAA
CCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGTACA
TGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACA
CTGGGCACCAAAGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGAGTC
GTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTACCC
TGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGGGAT
GAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTACCT
CACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTCAGC
CCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCA
GTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACCAGC
TCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCACAC
CCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGATGGC
TGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGGACC
GAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTCACC
CCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGC
CAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGC
CCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTTGAG
AAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATCCAA
CCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGAGCA
AGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCC
GTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCACCG
ACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCT
TTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAACCAA
GAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAATGG
TGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGCACA
AATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTC
TTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGA
GATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGGCGC
TGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAGGTT
CCCTATGTCCGATACACCATCCGCTGAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSSSS
PLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGL
YSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQ
GTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVED
FLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVAGTRGLFGQLFCDFGE
EMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQ
GMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFK
SLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEED
AAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGL
AAQEVMKACSGKFMPIMQWLYFDALECLPQDKEVLTEDKCLQRQNRYDGQ
VAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTD
MDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGP
DTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTK
GNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEG
LFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWA
DCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTF
DVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSG
VKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDD
SNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGL
VCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTL
WDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPA
AKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVR
YTIR*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5464
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with X56976 CDS: ; ; g569a (G190D), c1300g (Q434E)- these changes present in all E1a published sequences for all other species and in other human sequences (e.g. AAP36419, NP_695012) ; ; g1340a (R447H) – this change present in some mouse E1a sequences and in human EST sequences (e.g. IMAGE consortium EST clones 6537166, 6598339, 6464027).