cDNA Clone SIP9

• Plasmid: pGEX6P-3-SIP9
• Parent Plasmid: pGEX6P3
• Expressed: GST-SIP9
• Genbank: NM_025083
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCTACAGATTGGCTGGGAAGTATTGTGTCCATCAATTGTGGAGATAGCTTGGGTGTCTATCAGGGAAGAGTGTCAGCTGTGGATCAGGTCAGCCAGACCATTTCTCTCACCCGGCCTTTCCATAATGGAGTGAAGTGTCTTGTTCCAGAAGTCACCTTCAGGGCAGGTGACATTACGGAGTTAAAAATTCTGGAGATACCAGGACCTGGAGACAACCAACATTTTGGAGACCTTCATCAAACAGAATTAGGCCCCTCTGGTGCTGGCTGCCAAGTGGGCATCAATCAGAATGGCACA
GGCAAGTTTGTCAAGAAGCCAGCCTCTTCCAGCAGTGCCCCTCAGAATAT
CCCTAAGAGGACAGATGTGAAGAGCCAGGATGTTGCCGTTTCCCCGCAGC
AGCAACAGTGCTCAAAGAGCTATGTCGACAGGCACATGGAATCCTTGAGT
CAGTCCAAAAGTTTCCGTCGTCGGCACAACTCCTGGTCATCTAGTAGCAG
GCACCCAAATCAGGCAACTCCCAAGAAAAGTGGTTTAAAGAATGGCCAGA
TGAAGAATAAAGATGACGAGTGCTTCGGGGATGATATTGAGGAGATCCCA
GACACAGATTTTGATTTTGAAGGGAACCTGGCTCTTTTTGACAAGGCAGC
TGTGTTTGAGGAGATTGATACCTATGAAAGGAGAAGTGGTACCCGTTCCC
GGGGCATCCCAAATGAAAGGCCCACTCGGTACCGCCATGATGAGAACATC
TTGGAGTCCGAGCCCATTGTCTATCGACGGATCATAGTGCCCCACAACGT
GAGCAAGGAGTTCTGCACGGACTCTGGCCTGGTTGTCCCAAGTATTTCCT
ATGAGCTGCATAAAAAGCTGTTGTCCGTGGCTGAGAAGCATGGGCTGACC
CTTGAGCGGAGACTGGAGATGACAGGTGTGTGTGCCAGTCAGATGGCACT
GACCCTCCTCGGAGGACCTAACAGGTTGAATCCCAAAAATGTTCACCAGA
GGCCTACAGTGGCTCTACTGTGTGGACCTCATGTGAAGGGGGCTCAGGGT
ATCAGCTGTGGAAGGCACCTAGCCAACCATGATGTCCAGGTCATCCTTTT
CCTGCCCAATTTTGTCAAGATGTTGGAATCTATCACCAATGAGCTGTCGC
TCTTCAGCAAGACCCAAGGCCAACAAGTGTCTAGCCTCAAAGATCTGCCC
ACTAGCCCTGTGGACCTGGTCATCAACTGCCTGGATTGCCCTGAGAACGT
CTTCCTGCGCGATCAACCCTGGTACAAGGCAGCTGTGGCCTGGGCCAACC
AGAACCGGGCACCAGTACTCAGCATAGACCCTCCTGTGCATGAAGTCGAA
CAGGGCATTGATGCCAAATGGTCACTGGCACTGGGCCTGCCTCTGCCACT
GGGGGAGCACGCAGGCCGTATCTATTTGTGCGACATTGGCATTCCCCAGC
AGGTCTTCCAGGAGGTGGGCATCAACTACCACTCGCCCTTTGGCTGCAAG
TTTGTTATCCCACTGCACTCTGCTTAGggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMATDWLGSIVSINCGDSLG
VYQGRVSAVDQVSQTISLTRPFHNGVKCLVPEVTFRAGDITELKILEIPG
PGDNQHFGDLHQTELGPSGAGCQVGINQNGTGKFVKKPASSSSAPQNIPK
RTDVKSQDVAVSPQQQQCSKSYVDRHMESLSQSKSFRRRHNSWSSSSRHP
NQATPKKSGLKNGQMKNKDDECFGDDIEEIPDTDFDFEGNLALFDKAAVF
EEIDTYERRSGTRSRGIPNERPTRYRHDENILESEPIVYRRIIVPHNVSK
EFCTDSGLVVPSISYELHKKLLSVAEKHGLTLERRLEMTGVCASQMALTL
LGGPNRLNPKNVHQRPTVALLCGPHVKGAQGISCGRHLANHDVQVILFLP
NFVKMLESITNELSLFSKTQGQQVSSLKDLPTSPVDLVINCLDCPENVFL
RDQPWYKAAVAWANQNRAPVLSIDPPVHEVEQGIDAKWSLALGLPLPLGE
HAGRIYLCDIGIPQQVFQEVGINYHSPFGCKFVIPLHSA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 82848 Daltons