cDNA Clone PRAS40

• Plasmid: pGEX6P-3-PRAS40
• Parent Plasmid: pGEX6P3
• Expressed: GST-PRAS40
• Genbank: NM_032375
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCGTCGGGGCGCCCCGAGGAGCTGTGGGAGGCCGTGGTGGGGGCCGCCGAGCGCTTCCGGGCCCGGACTGGCACGGAGCTGGTGCTGCTGACCGCGGCCCCGCCGCCACCACCCCGCCCGGGCCCCTGTGCCTATCCTGCCCATGGTCGAGGAGCCCTGGCGGAGGCAGCGCGCCGTTGCCTCCACGACATCGCACTGGCCCACAGGGCTGCCACTGCTGCTCGGCCTCCTGCGCCCCCACCAGCACCACAGCCACCCAGTCCCACACCCAGCCCACCCCGGCCTACCCTGGCCAGA
GAGGACAACGAGGAGGACGAGGATGAGCCCACAGAGACAGAGACCTCCGG
GGAGCAGCTGGGCATTAGTGATAATGGAGGGCTCTTTGTGATGGATGAGG
ACGCCACCCTCCAGGACCTTCCCCCCTTCTGTGAGTCAGACCCCGAGAGT
ACAGATGATGGCAGCCTGAGCGAGGAGACCCCCGCCGGCCCCCCCACCTG
CTCAGTGCCCCCAGCCTCAGCCCTACCCACACAGCAGTACGCCAAGTCCC
TGCCTGTGTCTGTGCCCGTCTGGGGCTTCAAGGAGAAGAGGACAGAGGCG
CGGTCATCAGATGAGGAGAATGGGCCGCCCTCTTCGCCCGACCTGGACCG
CATCGCGGCGAGCATGCGCGCGCTGGTGCTGCGAGAGGCCGAGGACACCC
AGGTCTTCGGGGACCTGCCACGGCCGCGGCTTAACACCAGCGACTTCCAG
AAGCTGAAGCGGAAATATTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASGRPEELWEAVVGAAER
FRARTGTELVLLTAAPPPPPRPGPCAYPAHGRGALAEAARRCLHDIALAH
RAATAARPPAPPPAPQPPSPTPSPPRPTLAREDNEEDEDEPTETETSGEQ
LGISDNGGLFVMDEDATLQDLPPFCESDPESTDDGSLSEETPAGPPTCSV
PPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIA
ASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5458
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence difference compared with NM_032375 CDS:; t51c (silent A17); ; Estimated MW of expressed protein : 54199 Daltons