cDNA Clone EMAPL

• Plasmid: pGFP-TAP-BN EMAPL
• Parent Plasmid: pGFP TAP BN
• Expressed: GFP-TAP EMAPL
• Genbank: AK093146
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

gaattcTATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCGGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCC
ACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCT
CAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAAC
CTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCT
CTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAG
GCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATC
CCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGA
GACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGGGGTGG
CGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTG
TTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGAT
AAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGAC
GCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTG
GGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTG
GATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAAT
GAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCT
TCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTC
CACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCT
TACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCC
CTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAG
GGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGG
CAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTC
ATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTG
AGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGT
GGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCA
TTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCA
TTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCA
CACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCC
TCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCAT
GCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTT
CCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGT
TGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGC
AATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGC
CATTGGTTCCCATGACAACGTGATCTATATCTATAGTGTTTCCAGTGATG
GTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATC
ACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGG
GGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGA
AGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTG
CTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCC
GCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCAC
GACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCA
GTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCT
CTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTG
CCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCT
TCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGAC
TTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCC
CTGCACACACTGTTTAGgtcgac

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPLGSRSG
TAAARANSMELVKAALAEALRLLRLQVPPSSLQGSGTPAPPGDSLAAPPG
LPPTCTPSLVSRGTQTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQS
EGGGSSSSGAGSPGPPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKLS
RKAISSANLLVRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLR
GRPITMYIPSGIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSG
EVVYFIACVVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVA
SGQTAGVDKDGKPLQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAAD
QGAFLCVVDDSNEHMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCI
VTSGKSHVHFWNWSGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDG
DILTGDSEGNILTWGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFALC
LRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSEL
LVGTTKNALLRGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQL
CLWDGESHALAWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETRE
IVSDVIDGNEQLSVVRYSPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFGR
CMGHSSFITHLDWSKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRDR
EWATYTCVLGFHVYVPVRSCQGAEPHVRGPRQPRDQRPIHARRLAPRLAG
RQGRQHLPVASAGRWGRGAGARHALSNPLPVPRLLPRRLIAAWRDRLARR
RGPAPPCPSLAQSPTTRGRLFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5440
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; a126g (silent P41); a798g (silent G265); c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; c2031t (silent Y676); * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388