cDNA Clone EMAPL

• Plasmid: pCMV5-3HA EMAPL 1-344
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA EMAPL 1-344
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGT
GGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCC
ACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCT
CAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAAC
CTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCT
CTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAG
GCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATC
CCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGA
GACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGGGGTGG
CGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTG
TTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGAT
AAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGAC
GCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTG
GGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTTAGgaattc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMELVKAALAEALRLLRLQ
VPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSP
GPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQ
RADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLS
SPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEELPSGPPPET
LSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGGGG
QRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPVVHIWDSETL
LKLQEIGLGAFERGVGALAFSAADQG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5435
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; a798g (silent G266),; c867t (silent G289)*, ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; ; Estimated MW of expressed protein : 39454 Daltons