cDNA Clone EMAPL

• Plasmid: pCMV5-3HA EMAPL 355-end
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA EMAPL 355-end
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCTTCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAGGGGAACATTCTC
ACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGC
CAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTA
TCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGG
CGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGA
GGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGC
TTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGA
GACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCT
CTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCC
ACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGG
AGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGG
GGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACA
CAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTC
TCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGCCATTGGTTCCCA
TGACAACGTGATCTATATCTATAGTGTTTCCAGTGATGGTGCCAAATCCA
GCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGAC
TGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGAT
TCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATG
AGAGCCGAGACCGGGAATGGGCCACCTACACCTGTGTGCTGGGCTTTCAC
GTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCATGTACGGGG
GCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCACGACGACTCGCAC
CTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCAGTGGCGAGTGCT
GGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCTCTCGAACCCCCT
CCCTGTCCCCCGCCTCCTCCCTCGGCGTTTGATCGCTGCCTGGCGGGACC
GACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCTTCCCTGGCCCAA
TCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGACTTCGAGACATTC
CCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCCCTGCACACACTG
TTTAGgaattc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFEHMLSVWDCSRGMKLAEI
KSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGTLTRKQG
VFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTPGRGGAK
ETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGLVALQEA
EIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQGFSPVIQGHTDELW
GLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSIDLKETGLCADFHPSGA
VVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRYSPDGLHLAIGSHD
NVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNFIMSNSGDYEIL
YWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYVPVRSCQGAEPHVRGP
RQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGAGARHALSNPLP
VPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGRLFPGLTSRHSR
SRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5431
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; c2031t (silent Y677); t2238c (silent A746); a2490g (silent R830); * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 62192 Daltons