cDNA Clone EMAPL

• Plasmid: pCMV5-3HA EMAPL 258-end
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA EMAPL 258-end
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATGAGCACATG
CTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAGATCAAGAG
TACAAATGACTCAGTCTTGGCCGTTGGCTTCAACCCTCGTGACAGCAGCT
GCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATTGGAGTGGT
GGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAGGGTGTCTT
TGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTTCCTTCCGG
ATGGAGACATTCTCACCGGAGACTCAGAGGGGAACATTCTCACCTGGGGG
CGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCCAAAGAGAC
CTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTATCTTCGCCT
TGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGCGGGACCGC
CGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAGGCTGAGAT
TCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCTTGGCTCTG
AGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAGACCTGGCC
CAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTCTGGGGGCT
CTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCACGACCGGC
AGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGAGCATCGAC
CTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGGGCAGTTGT
GGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACACAGAGACCA
GAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTCTCAGTGGTC
CGGTACAGCCCAGATGGGTTGCACCTGGCCATTGGTTCCCATGACAACGT
GATCTATATCTATAGTGTTTCCAGTGATGGTGCCAAATCCAGCCGCTTTG
GCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGACTGGTCCAAG
GATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGATTCTTTACTG
GGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATGAGAGCCGAG
ACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTTTCACGTCTACGTA
CCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCATGTACGGGGGCCACGGCA
GCCACGTGACCAGCGTCCGATTCACGCACGACGACTCGCACCTCGTCTCG
CTGGGCGGCAAGGACGCCAGCATCTTCCAGTGGCGAGTGCTGGGCGCTGG
GGGCGCGGGGCCGGCGCCCGCCACGCCCTCTCGAACCCCCTCCCTGTCCC
CCGCCTCCTCCCTCGACGTTTGATCGCTGCCTGGCGGGACCGACTGGCCC
GGCGGCGTGGCCCCGCCCCGCCCTGCCCTTCCCTGGCCCAATCCCCCACG
ACTAGGGGCCGACTCTTTCCTGGACTGACTTCGAGACATTCCCGATCGCG
CATTTTCCTGGAGGGCGCGAACGGCGCCCCTGCACACACTGTTTAGgaattc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFGGGPGGPGGGGQRHYRGH
TDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPVVHIWDSETLLKLQEIG
LGAFERGVGALAFSAADQGAFLCVVDDSNEHMLSVWDCSRGMKLAEIKST
NDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGTLTRKQGVFG
KYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTPGRGGAKETY
GIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIP
EHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQGFSPVIQGHTDELWGLC
THPSQNRFLTCGHDRQLCLWDGESHALAWSIDLKETGLCADFHPSGAVVA
VGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRYSPDGLHLAIGSHDNVI
YIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNFIMSNSGDYEILYWD
VAGGCKQLKNRYESRDREWATYTCVLGFHVYVPVRSCQGAEPHVRGPRQP
RDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGAGARHALSNPLPVPR
LLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGRLFPGLTSRHSRSRI
FLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; c2031t (silent Y677); * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; ; Estimated MW of expressed protein : 72047 Daltons