cDNA Clone EMAPL

• Plasmid: pCMV5-3HA EMAPL 1-268
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA EMAPL 1-268
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGT
GGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCC
ACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCT
CAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAAC
CTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCT
CTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAG
GCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATC
CCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGA
GACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CCAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGG
CGGCTAGgaattc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMELVKAALAEALRLLRLQ
VPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKSSP
GPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQPPQ
RADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESRGGKDPLS
SPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEELPSGPPPET
LSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGGGG
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5429
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; t705c (silent in S235 (S234 in cloned fragment)); * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 31370 Daltons