cDNA Clone PP1 gamma

• Plasmid: pCW PP1 gamma GEFD-YRCG
• Parent Plasmid: pCW
• Expressed: PP1 gamma GEFD-YRCG
• Genbank: NM_002710
• Species: Human

Sequence of Insert

catatgGCGGATTTAGATAAACTCAACATCGACAGCATTATCCAACGGCTGCTGGAAGTGAGAGGGTCCAAGCCTGGTAAGAATGTCCAGCTTCAGGAGAATGAAATCAGAGGACTGTGCTTAAAGTCTCGTGAAATCTTTCTCAGTCAGCCT
ATCCTACTAGAACTTGAAGCACCACTCAAAATATGTGGTGACATCCATGG
ACAATACTATGATTTGCTGCGACTTTTTGAGTACGGTGGTTTCCCACCAG
AAAGCAACTACCTGTTTCTTGGGGACTATGTGGACAGGGGAAAGCAGTCA
TTGGAGACGATCTGCCTCTTACTGGCCTACAAAATAAAATATCCTGAGAA
TTTTTTTCTTCTCAGAGGGAACCATGAATGTGCCAGCATCAACAGAATTT
ATGGATTTTATGATGAATGTAAAAGAAGATACAACATTAAACTATGGAAA
ACTTTCACAGACTGTTTTAACTGTTTACCGATAGCAGCCATCGTGGATGA
GAAGATATTCTGCTGTCATGGAGGTTTATCACCAGATCTTCAATCTATGG
AGCAGATTCGGCGAATTATGCGACCAACTGATGTACCAGATCAAGGTCTT
CTTTGTGATCTTTTGTGGTCTGACCCCGATAAAGATGTCTTAGGCTGGGG
TGAAAATGACAGAGGAGTGTCCTTCACATTTGGTGCAGAAGTGGTTGCAA
AATTTCTCCATAAGCATGATTTGGATCTTATATGTAGAGCCCATCAGGTG
GTTGAAGATGGATATGAATTTTTTGCAAAGAGGCAGTTGGTCACTCTGTT
TTCTGCGCCCAATTATTGCTACCGGTGTGGCAATGCAGGTGCCATGATGA
GTGTGGATGAAACACTAATGTGTTCTTTTCAGATTTTAAAGCCTGCAGAG
AAAAAGAAGCCAAATGCCACGAGACCTGTAACGCCTCCAAGGGGTATGAT
CACAAAGCAAGCAAAGAAATAGgatccgcaagggcgaattctgcagatatccatcacactggcggccgctcgagcatgcatctaga

Amino Acid Sequence

MADLDKLNIDSIIQRLLEVRGSKPGKNVQLQENEIRGLCLKSREIFLSQP
ILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS
LETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWK
TFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGL
LCDLLWSDPDKDVLGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQV
VEDGYEFFAKRQLVTLFSAPNYCYRCGNAGAMMSVDETLMCSFQILKPAE
KKKPNATRPVTPPRGMITKQAKK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5407
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of untagged PP1 gamma G274Y, E275R, F276C, D277G.