cDNA Clone EMAPL

• Plasmid: pCMV5-3HA EMAPL
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA EMAPL
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcATGTACCCATACGATGTGCCAGATTACGCCGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCTTCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAGGGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGCAATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGCCATTGGTTCCCATGACAACGTGATCTACATCTATAGTGTTTCCAGTGATGGTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATCACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGAAGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTGCTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCCGCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCACGACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCAGTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCTCTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTGCCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCTTCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGACTTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCCCTGCACACACTGTTTAGgcggccgcacaagggcgaattc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFMYPYDVPDYAELVKAALA
EALRLLRLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTE
TEVELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPG
ILRPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTE
SRGGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEE
LPSGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGG
GPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPV
VHIWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEHMLS
VWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGV
GVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRS
PSDSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRL
VQWGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQG
FSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSIDLK
ETGLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRY
SPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDG
NFIMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYVPV
RSCQGAEPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGR
GAGARHALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTR
GRLFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5405
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 99856 Daltons