cDNA Clone Nur77 (mouse)

• Plasmid: pEBG6P-1 Nur77 S354D
• Parent Plasmid: pEBG6P
• Genbank: X60132
• Species: Mouse

Sequence of Insert

ggatccATGCCCTGTATTCAAGCTCAATATGGAACACCAGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTGATCCCCTGGCCCTTGAGTTCGGCAAGCCTACCATGGACCTGGCCAGCCCCGAGACAGCACCTACCGCACCTGCTACACTGCCCAGCTTCAGCACCTTCATGGACGGGTACACCGGAGAGTTTGACACCTTCCTCTACCAGCTGCCGGGGACGACCCAGCCGTGCTCCTCAGCTTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCTCGGCCACCTCCCCCGCTTCG
GCGTCCTTCAAGTTTGAGGACTTCCAGGTGTACGGCTGCTACCCGGGCAC
CCTGAGCGGCCCATTAGATGAGACCCTATCCTCCAGCGGCTCTGAGTACT
ATGGCAGTCCCTGCTCAGCCCCCTCGCCATCTACACCCAACTTCCAGCCG
TCCCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCTCCCCGAGCCA
GACTTATGAAGGCCTCTGGGCATGGACAGAGCAGTTGCCTAAGGCTTCTT
CAGGGCCCCCGCCACCTCCAACCTTCTTCTCCTTCAGTCCTCCCACTGGC
CCCAGCCCCAGCCTGGCCCAGAGTTCTCTGAAATTGTTCCCACCACCAGC
CACCCACCAGCTTGGGGAGGGGGAGAGCTATTCCATGCCAGCAGCTTTCC
CCGGCTTGGCACCCACCTCTCCGAACCGTGACACTTCCGGCATTCTGGAC
GCACCCGTGACCTCCACCAAGTCCCGGAGCGGGGCTTCAGGTGGCAGCGA
GGGCCGCTGTGCAGTCTGTGGTGACAATGCTTCGTGTCAGCACTATGGGG
TCCGCACCTGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTACAGAAA
AGCGCCAAGTACATCTGCCTGGCAAACAAGGATTGCCCTGTGGACAAGAG
GCGGCGGAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTGCCTGGCTGTGG
GCATGGTGAAGGAAGTTGTACGGACAGACAGCCTAAAAGGGCGGCGGGGC
CGGCTACCTGACAAACCCAAGCAGCCTCCAGATGCCTCCCCTACCAATCT
TCTCACTTCCCTCATCCGGGCACACTTGGACTCCGGGCCTAGCACTGCCA
AATTGGACTATTCCAAGTTCCAGGAACTGGTGCTGCCCCGCTTCGGGAAG
GAAGATGCCGGTGACGTGCAACAATTTTATGACTTGCTCTCTGGTTCCCT
GGACGTTATCCGAAAGTGGGCAGAAAAAATCCCTGGCTTCATTGAGCTTT
GCCCAGGAGACCAAGACCTGTTGCTAGAGTCTGCCTTCCTGGAACTCTTC
ATCCTCCGCCTGGCATACCGATCTAAACCCGGTGAGGGGAAGCTCATCTT
CTGCTCAGGCCTGGTACTACACCAGCTGCAGTGTGCCCGTGGCTTTGGTG
ATTGGATTGACAACATCCTGGCCTTCTCACGGTCCCTGCACAGCTTGGGT
GTTGATGTCCCCGCCTTTGCCTGCCTGTCCGCTCTGGTCCTCATCACTGA
TCGACACGGGCTCCAGGACCCTCGTCGGGTGGAAGAGCTGCAGAATCGCA
TTGCTAGCTGTCTGAAGGAGCACATGGCTACCGTGGCAGGGGACCCACAG
CCGGCCAGCTGCCTGTCACGTCTGCTGGGCAAACTGCCTGAGCTTCGGAC
CCTGTGCACTCAAGGCCTGCAGCGCATCTTTTGCCTCAAGTTGGAGGACT
TGGTACCCCCTCCACCTATTGTGGACAAGATCTTTATGGACACATTGTCT
TTCTGAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMPCIQAQYGTPAT
SPGPRDHLTGDPLALEFGKPTMDLASPETAPTAPATLPSFSTFMDGYTGE
FDTFLYQLPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGC
YPGTLSGPLDETLSSSGSEYYGSPCSAPSPSTPNFQPSQLSPWDGSFGHF
SPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSLKLF
PPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRSGAS
GGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKSAKYICLANKDCP
VDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPDKPKQPPDAS
PTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQFYDLL
SGSLDVIRKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSKPGEG
KLIFCSGLVLHQLQCARGFGDWIDNILAFSRSLHSLGVDVPAFACLSALV
LITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLLGKLP
ELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5402
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Nurr77 S354D with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with X60132 CDS:; g363a (silent P121), t558c (silent P186), t1509c (silent V503), a1641g (silent G547); plus: tca1060-1062gac (S354D); ; Estimated MW of expressed protein : 92155 Daltons