cDNA Clone F2KP (Arabidopsis)

• Plasmid: pCMV5-3HA At F2KP S303A
• Parent Plasmid: pCMV5 3HA
• Genbank: AF190739
• Tag: HA
• Species: Arabidopsis thaliana

Sequence of Insert

ggtaccATGGGGTCAGGTGCATCGAAGAATACTGAGGAAGATGACGATGGTTCCAATGGCGGAGGTGGTCAGCTCTACGTTTCACTCAAAATGGAAAACTCTAAGGTTGAAGGAGAGCTCACTCCTCATGTCTATGGCTCTCTTCCTCTCATCGGTTCCTGGGATCCTTCCAAAGCTCTTCCGATGCAACGTGAATCTGCGTTAATGTCGGAGCTAAGCTTCGTTGTTCCTCCTGATCACGAGACTTTGGACTTCAAGTTTTTGTTGAAGCCAAAGAACAGAAATACGCCCTGCATAGTGGAGGAG
GGTGAAAATAGGCTTCTTACTGGTGGTTCGTTGCAAGGAGATGCTAGATT
AGCTCTGTTTAGGCTGGAAGGTGATGTGATTGTTGAATTCCGAGTCTTCA
TCAACGCTGACAGAGTTTCTCCAATTGATCTTGCTACTAGTTGGAGAGCT
TATAGGGAGAACCTCCAGCCTTCTACTGTACGTGGAATTCCTGATGTCAG
CATTAATCCTGATCCAAAATCTGCTGAGTGTCCTTTAGAAAGTTTGGAGC
TCGATCTTGCACATTATGAAGTTCCTGCTCCTGCGCCTTCTGCAAACTCG
TACCTTGTTTATGCGGCGGACAATGCAGAGAATCCACGATCTCTTTCAGC
TTCTGGTTCATTTCGTAATGACAGTACTCCAAAAGCAGCACAGAGAAATT
CTGAAGATTCTGGTGTTACGGTTGATGGATCCCCATCTGCAAAGGAAATG
ACGATCGTTGTTCCTGATTCCTCGAATATCTATTCGGCTTTTGGAGAGGC
TGAGTCAAAGTCTGTGGAGACACTCTCACCGTTTCAGCAGAAAGATGGTC
AAAAGGGACTCTTTGTTGATCGTGGTGTTGGCTCCCCTAGGCTAGTGAAG
TCTCTTGCTGCAAGCTCTTTTTTGATTGATACCAAGCAAATCAAGAATTC
AATGCCAGCCGCTGCAGGAGCTGTTGCCGCAGCAGCGGTAGCTGATCAAA
TGCTTGGACCGAAAGAAGATAGACATCTAGCAATTGTCCTGGTTGGCTTG
CCTGCTCGAGGAAAGACTTTTACTGCAGCAAAGTTGACAAGATATCTACG
CTGGTTGGGACATGATACTAAACATTTTAATGTTGGAAAGTACCGACGGC
TTAAACATGGAGTTAACATGAGTGCTGATTTCTTCCGAGCTGATAATCCT
GAAGGTGTAGAGGCACGGACTGAGGTAGCAGCACTTGCTATGGAGGACAT
GATAGCTTGGATGCAAGAAGGTGGTCAAGTTGGAATCTTTGATGCAACTA
ATAGCACACGAGTTCGACGGAATATGTTGATGAAAATGGCTGAAGGAAAG
TGTAAGATCATTTTTCTGGAAACACTATGCAATGATGAGCGCATTATTGA
AAGAAACATACGTCTAAAAATCCAGCAAAGTCCTGACTATTCAGAAGAAA
TGGATTTTGAAGCTGGAGTACGAGACTTTAGAGACCGTTTAGCCAATTAT
GAAAAGGTTTATGAGCCTGTAGAAGAAGGTTCGTACATCAAAATGATTGA
TATGGTCAGTGGGAACGGTGGACAGATACAAGTGAACAATATTAGTGGTT
ACCTTCCTGGGAGAATTGTATTTTTCTTGGTGAATACGCATCTCACCCCA
CGACCAATACTACTCACTCGGCATGGAGAAAGCATGGATAATGTTAGAGG
CAGAATTGGAGGAGATAGCGTAATAAGTGACTCTGGTAAACTTTACGCAA
AGAAACTTGCCAGCTTTGTTGAAAAGCGACTTAAATCTGAAAAAGCTGCT
TCGATTTGGACCAGTACACTTCAGAGAACAAACCTAACAGCAAGTTCCAT
TGTCGGATTCCCCAAGGTGCAATGGCGTGCCCTGGACGAGATCAATGCTG
GAGTTTGTGATGGGATGACATATGAAGAGGTAAAGAAGAACATGCCAGAA
GAATACGAATCTCGAAAAAAGGACAAATTACGATACCGATACCCTCGTGG
AGAGTCCTACCTTGATGTAATCCAACGGCTTGAACCTGTGATCATTGAGT
TAGAACGACAAAGAGCTCCTGTCGTCGTGATATCTCACCAGGCTGTTCTG
AGAGCCTTGTATGCATATTTTGCGGATCGTCCCCTCAAAGAAATACCCCA
AATCGAGATGCCACTTCACACTATAATAGAGATACAGATGGGAGTTTCTG
GTGTGCAAGAGAAACGCTACAAACTCATGGACTGAgtcgac

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFRSGTMGSGASKNTEEDDD
GSNGGGGQLYVSLKMENSKVEGELTPHVYGSLPLIGSWDPSKALPMQRES
ALMSELSFVVPPDHETLDFKFLLKPKNRNTPCIVEEGENRLLTGGSLQGD
ARLALFRLEGDVIVEFRVFINADRVSPIDLATSWRAYRENLQPSTVRGIP
DVSINPDPKSAECPLESLELDLAHYEVPAPAPSANSYLVYAADNAENPRS
LSASGSFRNDSTPKAAQRNSEDSGVTVDGSPSAKEMTIVVPDSSNIYSAF
GEAESKSVETLSPFQQKDGQKGLFVDRGVGSPRLVKSLAASSFLIDTKQI
KNSMPAAAGAVAAAAVADQMLGPKEDRHLAIVLVGLPARGKTFTAAKLTR
YLRWLGHDTKHFNVGKYRRLKHGVNMSADFFRADNPEGVEARTEVAALAM
EDMIAWMQEGGQVGIFDATNSTRVRRNMLMKMAEGKCKIIFLETLCNDER
IIERNIRLKIQQSPDYSEEMDFEAGVRDFRDRLANYEKVYEPVEEGSYIK
MIDMVSGNGGQIQVNNISGYLPGRIVFFLVNTHLTPRPILLTRHGESMDN
VRGRIGGDSVISDSGKLYAKKLASFVEKRLKSEKAASIWTSTLQRTNLTA
SSIVGFPKVQWRALDEINAGVCDGMTYEEVKKNMPEEYESRKKDKLRYRY
PRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADRPLKE
IPQIEMPLHTIIEIQMGVSGVQEKRYKLMD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5396
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence changes compared with AF190739 CDS: ; c1886a (A629D - present in several other mRNAs) ; a907g/g908c (S303A); ; Estimated MW of expressed protein : 86811 Daltons