cDNA Clone Importin beta 1

• Plasmid: pGEX6P-3-Importin beta 1
• Parent Plasmid: pGEX6P3
• Expressed: GST-Importin beta 1
• Genbank: L38951
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTTGCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACTATGTTTTGCAGACATTGGGT
ACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTATTGC
TTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGG
TGGCCAATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACA
TTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACA
AGATAAATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAG
AAGAGCCTAGTAATAATGTGAAGCTAGCTGCTACGAATGCACTCCTGAAC
TCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAGTCTGAAAGGCACTT
TATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTAC
GAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAG
TACATGGAGACATATATGGGTCCTGCTCTTTTTGCAATCACAATCGAAGC
AATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATAGAATTCTGGT
CCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCA
GCAGAACAAGGACGGCCCCCTGAGCACACCAGCAAGTTTTATGCGAAGGG
AGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAAACAGG
ACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTG
TGCCTCATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGT
CCTCCCCTTCATTAAAGAACACATCAAGAACCCAGATTGGCGGTACCGGG
ATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACCAGAGCCCAGT
CAGCTCAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAAT
GAAAGACCCCAGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTAGGCA
GAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACTTGGCT
CCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGC
TTCAAATGTGTGCTGGGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAG
CTGCAGACGTTGCTGATGATCAGGAAGAACCAGCTACTTACTGCTTATCT
TCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACTACAGACAGACC
TGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGG
AAATTGTGAAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACG
ACTTTGGTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAGTCACA
TATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCTTTAC
TCTGTGCAACTCTTCAGAATGTTCTTCGGAAAGTGCAACATCAAGATGCT
TTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTTAAGGATGTTCCAAAG
CACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTTAGCA
CACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTT
AAACCCTTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTG
TTTGGCAGCTGTGGGCTTAGTGGGAGACTTGTGCCGTGCCCTGCAATCCA
ACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTGGAAAATTTG
GGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTT
TGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTAGAGG
TTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCA
GACTATGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGA
AGCCTATACTGGAATCGTCCAGGGATTAAAGGGGGATCAGGAGAACGTAC
ACCCGGATGTGATGCTGGTACAACCCAGAGTAGAATTTATTCTGTCTTTC
ATTGACCACATTGCTGGAGATGAGGATCACACAGATGGAGTAGTAGCTTG
TGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGATGTAC
TGAAATTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGG
CGGAGATCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAA
AGAACTGAGGAAACTGAAGAACCAAGCTTGAggatcc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMELITILEKTVSPDRLELE
AAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKNSLTSKD
PDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCVAGIACA
EIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDPEQLQDK
SNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESERHFIM
QVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMK
SDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGAL
QYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDIVPHVLP
FIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKD
PSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASN
VCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLETTDRPDG
HQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQMESHIQ
STSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTA
GSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLA
AVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQILSVFGD
IALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAY
TGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAA
GLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLATWATKEL
RKLKNQA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5345
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence differences compared with L38951 CDS: ; a585g (silent T195), c1632t (silent Y544); ; Estimated MW of expressed protein : 123914 Daltons