cDNA Clone Importin subunit alpha 1 (KPNA1)

• Plasmid: pGEX6P-3-Importin subunit alpha 1
• Parent Plasmid: pGEX6P3
• Expressed: GST-Importin subunit alpha 1
• Genbank: BT006959
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGACCACCCCAGGAAAAGAGAACTTTCGCCTGAAAAGTTACAAGAACAAATCTCTGAATCCCGATGAGATGCGCAGGAGGAGGGAGGAAGAAGGACTGCAGTTACGAAAGCAGAAAAGAGAAGAGCAGTTATTCAAGCGGAGAAATGTTGCTACAGCAGAAGAAGAAACAGAAGAAGAAGTTATGTCAGATGGAGGCTTTCATGAGGCTCAGATTAATAACATGGAGATGGCACCAGGTGGTGTCATCACTTCTGACATGATTGAAATGATATTTTCCAAAAGCCCAGAGCAACAGCTT
TCAGCAACACAGAAATTCAGGAAGCTGCTTTCAAAAGAACCTAACCCTCC
TATTGATGAAGTTATCAGCACACCAGGAGTAGTGGCCAGGTTTGTGGAGT
TCCTCAAACGAAAAGAGAATTGTACACTGCAGTTTGAATCAGCTTGGGTA
CTGACAAATATTGCTTCAGGAAATTCTCTTCAGACCCGAATTGTGATTCA
GGCAGGAGCTGTGCCCATCTTCATAGAGTTGCTCAGCTCAGAGTTTGAAG
ATGTCCAGGAACAGGCAGTCTGGGCTCTTGGCAACATTGCTGGAGATAGT
ACCATGTGCAGGGACTATGTCTTAGACTGCAATATCCTTCCCCCTCTTTT
GCAGTTATTTTCAAAGCAAAACCGCCTGACCATGACCCGGAATGCAGTAT
GGGCTTTGTCTAATCTCTGTAGAGGGAAAAGTCCACCTCCAGAATTTGCA
AAGGTTTCTCCATGTCTGAATGTGCTTTCCTGGTTGCTGTTTGTCAGTGA
CACTGATGTACTGGCTGATGCCTGCTGGGCCCTCTCATATCTATCAGATG
GACCCAATGATAAAATTCAAGCGGTCATCGATGCGGGAGTATGTAGGAGA
CTTGTGGAACTGCTGATGCATAATGATTATAAAGTGGTTTCTCCTGCTTT
GCGAGCTGTGGGAAACATTGTCACAGGGGATGATATTCAGACACAGGTAA
TTCTGAATTGCTCAGCTCTGCAGAGTTTATTGCATTTGCTGAGTAGCCCA
AAGGAATCTATCAAAAAGGAAGCATGTTGGACGATATCTAATATTACAGC
TGGAAATAGGGCACAGATCCAGACTGTGATAGATGCCAACATTTTCCCAG
CCCTCATTAGTATTTTACAAACTGCTGAATTTCGGACAAGAAAAGAAGCA
GCTTGGGCCATCACAAATGCAACTTCTGGAGGATCAGCTGAACAGATCAA
GTACCTAGTAGAACTGGGTTGTATCAAGCCGCTCTGTGATCTCCTCACGG
TCATGGACTCTAAGATTGTACAGGTTGCCCTAAATGGCTTGGAAAATATC
CTGAGGCTTGGAGAACAGGAAGCCAAAAGGAATGGCACTGGCATTAACCC
TTACTGTGCTTTGATTGAAGAAGCTTATGGTCTGGATAAAATTGAGTTCT
TACAGAGTCATGAAAACCAGGAGATCTACCAAAAGGCCTTTGATCTTATT
GAGCATTACTTCGGGACCGAAGATGAAGACAGCAGCATTGCACCCCAGGT
TGACCTTAACCAGCAGCAGTACATCTTCCAACAGTGTGAGGCTCCTATGG
AAGGTTTCCAGCTTTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTTPGKENFRLKSYKNKSL
NPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHE
AQINNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPID
EVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAG
AVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQL
FSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTD
VLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMHNDYKVVSPALRA
VGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKEACWTISNITAGN
RAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNATSGGSAEQIKYL
VELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQEAKRNGTGINPYC
ALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTEDEDSSIAPQVDL
NQQQYIFQQCEAPMEGFQL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5344
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 87017 Daltons