cDNA Clone Importin subunit alpha 4 (KPNA4)

• Plasmid: pGEX6P-3-importin subunit alpha 4
• Parent Plasmid: pGEX6P3
• Expressed: GST-importin subunit alpha 4
• Genbank: NM_002268
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGCGGACAACGAGAAACTGGACAACCAACGGCTCAAGAATTTCAAGAACAAAGGCCGCGACTTGGAGACTATGAGAAGGCAACGAAATGAAGTTGTAGTTGAATTAAGGAAGAATAAAAGAGATGAACATCTCTTAAAGAGAAGGAATGTACCACATGAAGATATCTGTGAAGACTCTGATATAGATGGTGATTATAGAGTGCAAAATACCTCTCTAGAAGCTATTGTTCAAAATGCTTCAAGTGATAACCAAGGAATTCAATTAAGTGCAGTTCAAGCTGCTAGGAAGCTTTTGTCC
AGTGATCGAAATCCACCAATTGATGACTTAATAAAATCTGGAATATTGCC
CATTTTAGTCCATTGTCTTGAAAGAGATGACAATCCTTCTTTACAGTTTG
AAGCTGCATGGGCTTTGACAAACATTGCATCTGGAACTTCTGAACAAACT
CAAGCAGTAGTTCAGTCCAATGCTGTGCCACTTTTCCTGAGGCTTCTCCA
TTCACCCCATCAGAATGTCTGTGAGCAAGCAGTGTGGGCATTGGGAAATA
TCATAGGTGATGGGCCCCAGTGTAGAGATTATGTCATAAGTCTTGGAGTT
GTGAAACCTTTACTTTCCTTCATAAGTCCATCTATTCCTATAACATTCTT
AAGAAATGTTACTTGGGTTATGGTCAACTTATGTCGCCACAAAGACCCAC
CACCACCAATGGAAACCATTCAGGAGATTCTTCCAGCCCTTTGTGTTTTA
ATTCATCACACAGATGTAAATATACTGGTAGACACAGTCTGGGCCCTCTC
TTACCTTACTGATGCTGGCAATGAACAAATACAGATGGTAATAGACTCTG
GAATAGTTCCTCATTTGGTTCCTCTGCTCAGCCACCAGGAAGTTAAAGTT
CAGACTGCTGCACTTAGAGCTGTGGGCAACATTGTTACTGGAACTGATGA
GCAAACACAAGTAGTTTTGAACTGTGATGCTCTTTCACACTTCCCAGCAC
TCCTGACACATCCCAAAGAGAAAATTAATAAAGAAGCAGTGTGGTTCCTC
TCCAACATCACTGCAGGAAATCAGCAGCAGGTACAGGCAGTAATTGATGC
CAATCTTGTACCAATGATAATACACCTTTTGGATAAGGGGGATTTTGGCA
CTCAAAAAGAAGCTGCTTGGGCCATAAGTAACTTAACAATTAGTGGAAGG
AAAGATCAAGTGGCTTACCTTATCCAACAAAATGTTATCCCACCTTTTTG
CAACTTGCTGACTGTAAAAGATGCACAAGTTGTGCAAGTAGTACTCGATG
GACTAAGTAATATATTAAAAATGGCTGAAGATGAGGCAGAAACCATAGGC
AATCTTATAGAAGAATGTGGAGGGCTGGAGAAAATTGAACAACTTCAAAA
TCATGAAAATGAAGACATCTACAAATTGGCCTATGAGATCATTGATCAGT
TCTTCTCTTCAGATGATATTGATGAAGACCCTAGCCTTGTTCCAGAGGCA
ATTCAAGGCGGAACATTTGGTTTCAATTCATCTGCCAATGTACCAACAGA
AGGGTTCCAGTTTTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADNEKLDNQRLKNFKNKG
RDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQ
NTSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPIL
VHCLERDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSP
HQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRN
VTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYL
TDAGNEQIQMVIDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQT
QVVLNCDALSHFPALLTHPKEKINKEAVWFLSNITAGNQQQVQAVIDANL
VPMIIHLLDKGDFGTQKEAAWAISNLTISGRKDQVAYLIQQNVIPPFCNL
LTVKDAQVVQVVLDGLSNILKMAEDEAETIGNLIEECGGLEKIEQLQNHE
NEDIYKLAYEIIDQFFSSDDIDEDPSLVPEAIQGGTFGFNSSANVPTEGF
QF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5343
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence difference compared with NM_002268 CDS:; a81g silent R27; ; Estimated MW of expressed protein : 84657 Daltons