cDNA Clone Importin beta 1

• Plasmid: pCMV5-3HA Importin beta 1
• Parent Plasmid: pCMV5 3HA
• Expressed: 3HA Importin beta 1
• Genbank: L38951
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTTGCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACTATGTTTTGCAGACATTGGGT
ACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTATTGC
TTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGG
TGGCCAATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACA
TTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACA
AGATAAATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAG
AAGAGCCTAGTAATAATGTGAAGCTAGCTGCTACGAATGCACTCCTGAAC
TCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAGTCTGAAAGGCACTT
TATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTAC
GAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAG
TACATGGAGACATATATGGGTCCTGCTCTTTTTGCAATCACAATCGAAGC
AATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATAGAATTCTGGT
CCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCA
GCAGAACAAGGACGGCCCCCTGAGCACACCAGCAAGTTTTATGCGAAGGG
AGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAAACAGG
ACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTG
TGCCTCATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGT
CCTCCCCTTCATTAAAGAACACATCAAGAACCCAGATTGGCGGTACCGGG
ATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACCAGAGCCCAGT
CAGCTCAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAAT
GAAAGACCCCAGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTAGGCA
GAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACTTGGCT
CCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGC
TTCAAATGTGTGCTGGGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAG
CTGCAGACGTTGCTGATGATCAGGAAGAACCAGCTACTTACTGCTTATCT
TCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACTACAGACAGACC
TGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGG
AAATTGTGAAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACG
ACTTTGGTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAGTCACA
TATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCTTTAC
TCTGTGCAACTCTTCAGAATGTTCTTCGGAAAGTGCAACATCAAGATGCT
TTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTTAAGGATGTTCCAAAG
CACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTTAGCA
CACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTT
AAACCCTTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTG
TTTGGCAGCTGTGGGCTTAGTGGGAGACTTGTGCCGTGCCCTGCAATCCA
ACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTGGAAAATTTG
GGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTT
TGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTAGAGG
TTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCA
GACTATGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGA
AGCCTATACTGGAATCGTCCAGGGATTAAAGGGGGATCAGGAGAACGTAC
ACCCGGATGTGATGCTGGTACAACCCAGAGTAGAATTTATTCTGTCTTTC
ATTGACCACATTGCTGGAGATGAGGATCACACAGATGGAGTAGTAGCTTG
TGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGATGTAC
TGAAATTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGG
CGGAGATCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAA
AGAACTGAGGAAACTGAAGAACCAAGCTTGAggatcc

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAEFRSGTTRIDKLACLQVDSR
GSMELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPG
NSQVARVAAGLQIKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQT
LGTETYRPSSASQCVAGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKE
STLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNAL
LNSLEFTKANFDKESERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLY
YQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEAS
EAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAA
GVCLMLLATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPE
PSQLKPLVIQAMPTLIELMKDPSVVVRDTAAWTVGRICELLPEAAINDVY
LAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPATYC
LSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQ
KTTLVIMERLQQVLQMESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQ
DALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYME
AFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLE
NLGNENVHRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVD
KSDYDMVDYLNELRESCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFIL
SFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLT
EGRRSKTNKAKTLATWATKELRKLKNQA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5332
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with L38951 CDS: ; a585g (silent T195), c1632t (silent Y544); ; Estimated MW of expressed protein : 103171 Daltons