cDNA Clone Importin beta 1

• Plasmid: pEBG6P-1 Importin beta 1
• Parent Plasmid: pEBG6P
• Expressed: GST-Importin beta 1
• Genbank: L38951
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTTGCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACTATGTTTTGCAGACATTGGGT
ACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTATTGC
TTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGG
TGGCCAATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACA
TTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACA
AGATAAATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAG
AAGAGCCTAGTAATAATGTGAAGCTAGCTGCTACGAATGCACTCCTGAAC
TCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAGTCTGAAAGGCACTT
TATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTAC
GAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAG
TACATGGAGACATATATGGGTCCTGCTCTTTTTGCAATCACAATCGAAGC
AATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATAGAATTCTGGT
CCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCA
GCAGAACAAGGACGGCCCCCTGAGCACACCAGCAAGTTTTATGCGAAGGG
AGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAAACAGG
ACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTG
TGCCTCATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGT
CCTCCCCTTCATTAAAGAACACATCAAGAACCCAGATTGGCGGTACCGGG
ATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACCAGAGCCCAGT
CAGCTCAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAAT
GAAAGACCCCAGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTAGGCA
GAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACTTGGCT
CCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGC
TTCAAATGTGTGCTGGGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAG
CTGCAGACGTTGCTGATGATCAGGAAGAACCAGCTACTTACTGCTTATCT
TCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACTACAGACAGACC
TGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGG
AAATTGTGAAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACG
ACTTTGGTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAGTCACA
TATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCTTTAC
TCTGTGCAACTCTTCAGAATGTTCTTCGGAAAGTGCAACATCAAGATGCT
TTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTTAAGGATGTTCCAAAG
CACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTTAGCA
CACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTT
AAACCCTTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTG
TTTGGCAGCTGTGGGCTTAGTGGGAGACTTGTGCCGTGCCCTGCAATCCA
ACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTGGAAAATTTG
GGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTT
TGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTAGAGG
TTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCA
GACTATGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGA
AGCCTATACTGGAATCGTCCAGGGATTAAAGGGGGATCAGGAGAACGTAC
ACCCGGATGTGATGCTGGTACAACCCAGAGTAGAATTTATTCTGTCTTTC
ATTGACCACATTGCTGGAGATGAGGATCACACAGATGGAGTAGTAGCTTG
TGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGATGTAC
TGAAATTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGG
CGGAGATCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAA
AGAACTGAGGAAACTGAAGAACCAAGCTTGAggatcc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMELITILEKTVSP
DRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQIKN
SLTSKDPDIKAQYQQRWLAIDANARREVKNYVLQTLGTETYRPSSASQCV
AGIACAEIPVNQWPELIPQLVANVTNPNSTEHMKESTLEAIGYICQDIDP
EQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKES
ERHFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAI
TIEAMKSDIDEVALQGIEFWSNVCDEEMDLAIEASEAAEQGRPPEHTSKF
YAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLLATCCEDDI
VPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTL
IELMKDPSVVVRDTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAE
PRVASNVCWAFSSLAEAAYEAADVADDQEEPATYCLSSSFELIVQKLLET
TDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQ
MESHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLR
MFQSTAGSGGVQEDALMAVSTLVEVLGGEFLKYMEAFKPFLGIGLKNYAE
YQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENVHRSVKPQI
LSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRE
SCLEAYTGIVQGLKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDG
VVACAAGLIGDLCTAFGKDVLKLVEARPMIHELLTEGRRSKTNKAKTLAT
WATKELRKLKNQA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5331
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express Importin beta 1 with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with L38951 CDS: ; a585g (silent T195), c1632t (silent Y544); ; Estimated MW of expressed protein : 124508 Daltons