cDNA Clone HIPK2

• Plasmid: pCMV5-HA HIPK2
• Parent Plasmid: pCMV5
• Expressed: HA-HIPK2
• Genbank: AF326592
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTGCCTTCTGTAGTGTGAAGAAACTGAAAATAGAGCCGAGTTCCAACTGGGACATGACTGGGTACGGCTCCCACAGCAAAGTGTATAGCCAGAGCAAGAACATCCCCCTGTCGCAGCCAGCCACCACAACCGTCAGCACCTCCTTGCCGGTCCCAAACCCAAGCCTACCTTACGAGCAGACCATCGTCTTCCCAGGAAGCACCGGGCACATCGTGGTCACCTCAGCAAGCAGCACTTCTGTCACCGGGCAAGTCCTCGGCGGACCACACAACCTAATGCGTCGAAGCACTGTGAGCCTCCTTGATACCTACCAAAAATGTGGACTCAAGCGTAAGAGCGAGGAGATCGAGAACACAAGCAGCGTGCAGATCATCGAGGAGCATCCACCCATGATTCAGAATAATGCAAGCGGGGCCACTGTCGCCACTGCCACCACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACTATCAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAGGTCTTAGAGTTCTTGGGCCGAGGGACGTTTGGGCAAGTGGTCAAGTGCTGGAAACGGGGCACCAATGAGATCGTGGCCATCAAGATCCTGAAGAACCACCCATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTGAGCACGGAGAGTGCCGATGACTATAACTTCGTCCGGGCCTACGAATGCTTCCAGCACAAGAACCACACGTGCTTGGTCTTCGAGATGTTGGAGCAGAACCTCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCCTCAAATACATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAGCCTAGGTCTTATCCACGCTGACCTCAAACCAGAAAACATCATGCTGGTGGATCCATCTAGACAACCATACAGAGTCAAGGTCATCGACTTTGGTTCAGCCAGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCCAGATATTACAGGGCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGCAATTGACATGTGGTCCCTGGGCTGTGTTATTGCAGAATTGTTCCTGGGTTGGCCGTTATATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCACAAACACAGGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGACAACTAGGTTTTTCAACCGTGACACGGACTCACCATATCCTTTGTGGAGACTGAAGACACCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAAGTACATTTTCATCTGTTTAGATGATATGGCCCAGGTGAACATGACGACAGATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTCATTGACCTGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCACTCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTACTCGATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATCTGCAAGCGTCGGGTGAATATGTATGACACGGTGAACCAGAGCAAAACCCCTTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCTTTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATGGCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGATTTGTGCCCGGCCTGACCCGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCGGCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTGCGAATGGAAAATGCAGTTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCCTCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGACCCAGCAGATCCTACTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCC
ACCCACACATCAGTGCAGCATGCCACCGTGATTCCCGAGACCATGGCAGG
CACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATT
ATAATCCCATCATGCAGCAGCCTGCACTATTGACCGGTCATGTGACCCTT
CCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCA
GCCAACCAGCACCACCTCCTCCCGGAAGAGTAAGCAGCACCAGTCATCTG
TGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCC
CCACAGCGATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCAT
GGTGCACAGTAGCCCGGCCTGCAGCACCTCGGTCACCTGCGGGTGGGGCG
ACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACAATTGTCATT
CCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTGACAC
GGACGAGGAGGAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGC
AAAGAAAAAACGTCATCAGCTGTGTCACAGTCCACGACTCCCCCTACTCC
GACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCA
CAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCA
CGGGGAACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGC
GAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGTCAACACCAGTCACCA
CTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCG
GTCACTCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGG
CCGGGCCCCCACTTCCAGCAGCAGCAGCCACTCAATCTCAGCCAGGCTCA
GCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAGCAGGCCT
ACATCACTCCCACCATGGCCCAGGCTCCGTACTCCTTCCCGCACAACAGC
CCCAGCCACGGCACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGC
CCACCTCCCCACCCAGCCCCACCTCTACACCTACACTGCGCCGGCGGCCC
TGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCG
CGCCACACCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGT
CCCCGTGAGCATGGGCCCCCGGGTCCTGCCCTCGCCCACCATCCACCCGA
GTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATCAGCGCCTCGCCA
GCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCAACCA
GTACCCTTACATATAAaagctt

Amino Acid Sequence

MYPYDVPDYAMAPVYEGMASHVQVFSPHTLQSSAFCSVKKLKIEPSSNWD
MTGYGSHSKVYSQSKNIPLSQPATTTVSTSLPVPNPSLPYEQTIVFPGST
GHIVVTSASSTSVTGQVLGGPHNLMRRSTVSLLDTYQKCGLKRKSEEIEN
TSSVQIIEEHPPMIQNNASGATVATATTSTATSKNSGSNSEGDYQLVQHE
VLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQG
QIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQ
NKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPY
RVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCV
IAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTD
SPYPLWRLKTPDDHEAETGIKSKEARKYIFICLDDMAQVNMTTDLEGSDM
LVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDFPHSTH
VKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMTFNNQLTT
VHNQPSAASMAAVAQRSMPLQTGTAQICARPDPFQQALIVCPPGFQGLQA
SPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGLLAQQAWPSGTQQILLP
PAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGSHYNPIMQQ
PALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSVRNVSTC
EVSSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTT
RERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVIS
CVTVHDSPYSDSSSNTSPYSVQQRAGHNNANAFDTKGSLENHCTGNPRTI
IVPPLKTQASEVLVECDSLVPVNTSHHSSSYKSKSSSNVTSTSGHSSGSS
SGAITYRQQRPGPHFQQQQPLNLSQAQQHITTDRTGSHRRQQAYITPTMA
QAPYSFPHNSPSHGTVHPHLAAAAAAAHLPTQPHLYTYTAPAALGSTGTV
AHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQF
AHQTYISASPASTVYTGYPLSPAKVNQYPYI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5320
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AF326592 CDS: ; a675g (silent V225), g2064a (silent L688), t2490c (silent C830); ; Estimated MW of expressed protein : 129291 Daltons