cDNA Clone HIPK2

• Plasmid: pFastBac.HTa HIPK2
• Parent Plasmid: pSC-A
• Expressed: 6His-HIPK2
• Genbank: AF326592
• Tag: 6His
• Species: Human

Sequence of Insert

gaattcATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTGCCTTCTGTAGTGTGAAGAAACTGAAAATAGAGCCGAGTTCCAACTGGGACATGACTGGGTACGGCTCCCACAGCAAAGTGTATAGCCAGAGCAAGAACATCCCCCTGTCGCAGCCAGCCACCACAACCGTCAGCACCTCCTTGCCGGTCCCAAACCCAAGCCTACCTTACGAGCAGACCATCGTCTTCCCAGGAAGCACCGGGCACATCGTGGTCACCTCAGCAAGCAGC
ACTTCTGTCACCGGGCAAGTCCTCGGCGGACCACACAACCTAATGCGTCG
AAGCACTGTGAGCCTCCTTGATACCTACCAAAAATGTGGACTCAAGCGTA
AGAGCGAGGAGATCGAGAACACAAGCAGCGTGCAGATCATCGAGGAGCAT
CCACCCATGATTCAGAATAATGCAAGCGGGGCCACTGTCGCCACTGCCAC
CACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACT
ATCAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAG
GTCTTAGAGTTCTTGGGCCGAGGGACGTTTGGGCAAGTGGTCAAGTGCTG
GAAACGGGGCACCAATGAGATCGTGGCCATCAAGATCCTGAAGAACCACC
CATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTG
AGCACGGAGAGTGCCGATGACTATAACTTCGTCCGGGCCTACGAATGCTT
CCAGCACAAGAACCACACGTGCTTGGTCTTCGAGATGTTGGAGCAGAACC
TCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCCTCAAATAC
ATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAG
CCTAGGTCTTATCCACGCTGACCTCAAACCAGAAAACATCATGCTGGTGG
ATCCATCTAGACAACCATACAGAGTCAAGGTCATCGACTTTGGTTCAGCC
AGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCCAGATATTA
CAGGGCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGCAATTGACA
TGTGGTCCCTGGGCTGTGTTATTGCAGAATTGTTCCTGGGTTGGCCGTTA
TATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCACAAACACA
GGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGACAACTAGGT
TTTTCAACCGTGACACGGACTCACCATATCCTTTGTGGAGACTGAAGACA
CCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAA
GTACATTTTCATCTGTTTAGATGATATGGCCCAGGTGAACATGACGACAG
ATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTC
ATTGACCTGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCAC
TCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTACTCG
ATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATC
TGCAAGCGTCGGGTGAATATGTATGACACGGTGAACCAGAGCAAAACCCC
TTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCT
TTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATG
GCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGAT
TTGTGCCCGGCCTGACCCGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCG
GCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTG
CGAATGGAAAATGCAGTTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCC
TCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGA
CCCAGCAGATCCTACTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCC
ACCCACACATCAGTGCAGCATGCCACCGTGATTCCCGAGACCATGGCAGG
CACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATT
ATAATCCCATCATGCAGCAGCCTGCACTATTGACCGGTCATGTGACCCTT
CCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCA
GCCAACCAGCACCACCTCCTCCCGGAAGAGTAAGCAGCACCAGTCATCTG
TGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCC
CCACAGCGATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCAT
GGTGCACAGTAGCCCGGCCTGCAGCACCTCGGTCACCTGTGGGTGGGGCG
ACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACAATTGTCATT
CCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTGACAC
GGACGAGGAGGAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGC
AAAGAAAAAACGTCATCAGCTGTGTCACAGTCCACGACTCCCCCTACTCC
GACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCA
CAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCA
CGGGGAACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGC
GAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGTCAACACCAGTCACCA
CTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCG
GTCACTCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGG
CCGGGCCCCCACTTCCAGCAGCAGCAGCCACTCAATCTCAGCCAGGCTCA
GCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAGCAGGCCT
ACATCACTCCCACCATGGCCCAGGCTCCGTACTCCTTCCCGCACAACAGC
CCCAGCCACGGCACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGC
CCACCTCCCCACCCAGCCCCACCTCTACACCTACACTGCGCCGGCGGCCC
TGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCG
CGCCACACCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGT
CCCCGTGAGCATGGGCCCCCGGGTCCTGCCCTCGCCCACCATCCACCCGA
GTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATCAGCGCCTCGCCA
GCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCAACCA
GTACCCTTACATATAAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFMAPVYEGMASHVQVFSPHTL
QSSAFCSVKKLKIEPSSNWDMTGYGSHSKVYSQSKNIPLSQPATTTVSTS
LPVPNPSLPYEQTIVFPGSTGHIVVTSASSTSVTGQVLGGPHNLMRRSTV
SLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTST
ATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRG
TNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHK
NHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGL
IHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAP
EIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLP
AEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIF
ICLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIE
TLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFIT
HVAPSTSTNLTMTFNNQLTTVHNQPSAASMAAVAQRSMPLQTGTAQICAR
PDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQI
QPGLLAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVIPETMAGTQQ
LADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTS
TTSSRKSKQHQSSVRNVSTCEVSSSQAISSPQRSKRVKENTPPRCAMVHS
SPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDTDEE
EEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSVQQRAGHNNA
NAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVPVNTSHHSSS
YKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQQPLNLSQAQQHI
TTDRTGSHRRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLAAAAAAAHLP
TQPHLYTYTAPAALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQVPVS
MGPRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAKVNQYPY
I*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5315
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag.; N.B. Sequence differences compared with AF326592 CDS: ; a675g (silent V225), g2064a (silent L688); ; Estimated MW of expressed protein : 131790 Daltons