cDNA Clone HIPK2

• Plasmid: pEBG6P-1 HIPK2
• Parent Plasmid: pEBG6P
• Expressed: GST-HIPK2
• Genbank: AF326592
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGGCCCCCGTGTACGAAGGTATGGCCTCACATGTGCAAGTTTTCTCCCCTCACACCCTTCAATCAAGTGCCTTCTGTAGTGTGAAGAAACTGAAAATAGAGCCGAGTTCCAACTGGGACATGACTGGGTACGGCTCCCACAGCAAAGTGTATAGCCAGAGCAAGAACATCCCCCTGTCGCAGCCAGCCACCACAACCGTCAGCACCTCCTTGCCGGTCCCAAACCCAAGCCTACCTTACGAGCAGACCATCGTCTTCCCAGGAAGCACCGGGCACATCGTGGTCACCTCAGCAAGCAGC
ACTTCTGTCACCGGGCAAGTCCTCGGCGGACCACACAACCTAATGCGTCG
AAGCACTGTGAGCCTCCTTGATACCTACCAAAAATGTGGACTCAAGCGTA
AGAGCGAGGAGATCGAGAACACAAGCAGCGTGCAGATCATCGAGGAGCAT
CCACCCATGATTCAGAATAATGCAAGCGGGGCCACTGTCGCCACTGCCAC
CACGTCTACTGCCACCTCCAAAAACAGCGGCTCCAACAGCGAGGGCGACT
ATCAGCTGGTGCAGCATGAGGTGCTGTGCTCCATGACCAACACCTACGAG
GTCTTAGAGTTCTTGGGCCGAGGGACGTTTGGGCAAGTGGTCAAGTGCTG
GAAACGGGGCACCAATGAGATCGTGGCCATCAAGATCCTGAAGAACCACC
CATCCTATGCCCGACAAGGTCAGATTGAAGTGAGCATCCTGGCCCGGTTG
AGCACGGAGAGTGCCGATGACTATAACTTCGTCCGGGCCTACGAATGCTT
CCAGCACAAGAACCACACGTGCTTGGTCTTCGAGATGTTGGAGCAGAACC
TCTATGACTTTCTGAAGCAAAACAAGTTTAGCCCCTTGCCCCTCAAATAC
ATTCGCCCAGTTCTCCAGCAGGTAGCCACAGCCCTGATGAAACTCAAAAG
CCTAGGTCTTATCCACGCTGACCTCAAACCAGAAAACATCATGCTGGTGG
ATCCATCTAGACAACCATACAGAGTCAAGGTCATCGACTTTGGTTCAGCC
AGCCACGTCTCCAAGGCTGTGTGCTCCACCTACTTGCAGTCCAGATATTA
CAGGGCCCCTGAGATCATCCTTGGTTTACCATTTTGTGAGGCAATTGACA
TGTGGTCCCTGGGCTGTGTTATTGCAGAATTGTTCCTGGGTTGGCCGTTA
TATCCAGGAGCTTCGGAGTATGATCAGATTCGGTATATTTCACAAACACA
GGGTTTGCCTGCTGAATATTTATTAAGCGCCGGGACAAAGACAACTAGGT
TTTTCAACCGTGACACGGACTCACCATATCCTTTGTGGAGACTGAAGACA
CCAGATGACCATGAAGCAGAGACAGGGATTAAGTCAAAAGAAGCAAGAAA
GTACATTTTCATCTGTTTAGATGATATGGCCCAGGTGAACATGACGACAG
ATTTGGAAGGGAGCGACATGTTGGTAGAAAAGGCTGACCGGCGGGAGTTC
ATTGACCTGTTGAAGAAGATGCTGACCATTGATGCTGACAAGAGAATCAC
TCCAATCGAAACCCTGAACCATCCCTTTGTCACCATGACACACTTACTCG
ATTTTCCCCACAGCACACACGTCAAATCATGTTTCCAGAACATGGAGATC
TGCAAGCGTCGGGTGAATATGTATGACACGGTGAACCAGAGCAAAACCCC
TTTCATCACGCACGTGGCCCCCAGCACGTCCACCAACCTGACCATGACCT
TTAACAACCAGCTGACCACTGTCCACAACCAGCCCTCAGCGGCATCCATG
GCTGCAGTGGCCCAGCGGAGCATGCCCCTGCAGACAGGAACAGCCCAGAT
TTGTGCCCGGCCTGACCCGTTCCAGCAAGCTCTCATCGTGTGTCCCCCCG
GCTTCCAAGGCTTGCAGGCCTCTCCCTCTAAGCACGCTGGCTACTCGGTG
CGAATGGAAAATGCAGTTCCCATCGTCACTCAAGCCCCAGGAGCTCAGCC
TCTTCAGATCCAACCAGGTCTGCTTGCCCAGCAGGCTTGGCCAAGTGGGA
CCCAGCAGATCCTACTTCCCCCAGCATGGCAGCAACTGACTGGAGTGGCC
ACCCACACATCAGTGCAGCATGCCACCGTGATTCCCGAGACCATGGCAGG
CACCCAGCAGCTGGCGGACTGGAGAAATACGCATGCTCACGGAAGCCATT
ATAATCCCATCATGCAGCAGCCTGCACTATTGACCGGTCATGTGACCCTT
CCAGCAGCACAGCCCTTAAATGTGGGTGTGGCCCACGTGATGCGGCAGCA
GCCAACCAGCACCACCTCCTCCCGGAAGAGTAAGCAGCACCAGTCATCTG
TGAGAAATGTCTCCACCTGTGAGGTGTCCTCCTCTCAGGCCATCAGCTCC
CCACAGCGATCCAAGCGTGTCAAGGAGAACACACCTCCCCGCTGTGCCAT
GGTGCACAGTAGCCCGGCCTGCAGCACCTCGGTCACCTGTGGGTGGGGCG
ACGTGGCCTCCAGCACCACCCGGGAACGGCAGCGGCAGACAATTGTCATT
CCCGACACTCCCAGCCCCACGGTCAGCGTCATCACCATCAGCAGTGACAC
GGACGAGGAGGAGGAACAGAAACACGCCCCCACCAGCACTGTCTCCAAGC
AAAGAAAAAACGTCATCAGCTGTGTCACAGTCCACGACTCCCCCTACTCC
GACTCCTCCAGCAACACCAGCCCCTACTCCGTGCAGCAGCGTGCTGGGCA
CAACAATGCCAATGCCTTTGACACCAAGGGGAGCCTGGAGAATCACTGCA
CGGGGAACCCCCGAACCATCATCGTGCCACCCCTGAAAACCCAGGCCAGC
GAAGTATTGGTGGAGTGTGATAGCCTGGTGCCAGTCAACACCAGTCACCA
CTCGTCCTCCTACAAGTCCAAGTCCTCCAGCAACGTGACCTCCACCAGCG
GTCACTCTTCAGGGAGCTCATCTGGAGCCATCACCTACCGGCAGCAGCGG
CCGGGCCCCCACTTCCAGCAGCAGCAGCCACTCAATCTCAGCCAGGCTCA
GCAGCACATCACCACGGACCGCACTGGGAGCCACCGAAGGCAGCAGGCCT
ACATCACTCCCACCATGGCCCAGGCTCCGTACTCCTTCCCGCACAACAGC
CCCAGCCACGGCACTGTGCACCCGCATCTGGCTGCAGCCGCTGCCGCTGC
CCACCTCCCCACCCAGCCCCACCTCTACACCTACACTGCGCCGGCGGCCC
TGGGCTCCACCGGCACCGTGGCCCACCTGGTGGCCTCGCAAGGCTCTGCG
CGCCACACCGTGCAGCACACTGCCTACCCAGCCAGCATCGTCCACCAGGT
CCCCGTGAGCATGGGCCCCCGGGTCCTGCCCTCGCCCACCATCCACCCGA
GTCAGTATCCAGCCCAATTTGCCCACCAGACCTACATCAGCGCCTCGCCA
GCCTCCACCGTCTACACTGGATACCCACTGAGCCCCGCCAAGGTCAACCA
GTACCCTTACATATAAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMAPVYEGMASH
VQVFSPHTLQSSAFCSVKKLKIEPSSNWDMTGYGSHSKVYSQSKNIPLSQ
PATTTVSTSLPVPNPSLPYEQTIVFPGSTGHIVVTSASSTSVTGQVLGGP
HNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGA
TVATATTSTATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTFG
QVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFV
RAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATA
LMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTY
LQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIR
YISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIK
SKEARKYIFICLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTID
ADKRITPIETLNHPFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTV
NQSKTPFITHVAPSTSTNLTMTFNNQLTTVHNQPSAASMAAVAQRSMPLQ
TGTAQICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQ
APGAQPLQIQPGLLAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVI
PETMAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVA
HVMRQQPTSTTSSRKSKQHQSSVRNVSTCEVSSSQAISSPQRSKRVKENT
PPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIPDTPSPTVSVI
TISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYSV
QQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVP
VNTSHHSSSYKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQQPL
NLSQAQQHITTDRTGSHRRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLA
AAAAAAHLPTQPHLYTYTAPAALGSTGTVAHLVASQGSARHTVQHTAYPA
SIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLS
PAKVNQYPYI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5313
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express HIPK2 with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with AF326592 CDS: ; a675g (silent V225), g2064a (silent L688); ; Estimated MW of expressed protein : 155664 Daltons