cDNA Clone BAD (mouse)

• Plasmid: pEBG6P-1 (mouse) BAD S136E
• Parent Plasmid: pEBG6P
• Genbank: BC006762
• Species: Mouse

Sequence of Insert

ggatccATGGGAACCCCAAAGCAGCCCTCGCTGGCTCCTGCACACGCCCTAGGCTTGAGGAAGTCCGATCCCGGAATCCGGAGCCTGGGGAGCGACGCGGGAGGAAGGCGGTGGAGACCAGCAGCCCAGAGTATGTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAAGACGCTAGTGCTACAGATAGGGGCCTGGGCCCTAGCCTCACTGAGGACCAGCCAGGTCCCTACCTGGCCCCAGGTCTCCTGGGGAGCAACATTCATCAGCAGGGACGGGCAGCCACCAACAGTCATCATGGAGGC
GCAGGGGCTATGGAGACTCGGAGTCGCCACAGTTCGTACCCAGCGGGGAC
CGAGGAGGATGAAGGGATGGAGGAGGAGCTTAGCCCTTTTCGAGGACGCT
CGCGTGAGGCTCCCCCCAATCTCTGGGCAGCGCAGCGCTACGGCCGTGAG
CTCCGAAGGATGAGCGATGAGTTTGAGGGTTCCTTCAAGGGACTTCCTCG
CCCAAAGAGCGCAGGCACTGCAACACAGATGCGACAAAGCGCCGGCTGGA
CGCGCATTATCCAGTCCTGGTGGGATCGAAACTTGGGCAAAGGAGGCTCC
ACCCCCTCCCAGTGAgcggccgc

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMGTPKQPSLAPAH
ALGLRKSDPGIRSLGSDAGGRRWRPAAQSMFQIPEFEPSEQEDASATDRG
LGPSLTEDQPGPYLAPGLLGSNIHQQGRAATNSHHGGAGAMETRSRHSSY
PAGTEEDEGMEEELSPFRGRSREAPPNLWAAQRYGRELRRMSDEFEGSFK
GLPRPKSAGTATQMRQSAGWTRIIQSWWDRNLGKGGSTPSQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5310
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express mouse BAD S136E with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 49508 Daltons