cDNA Clone HIPK3

• Plasmid: pCMV5-HA HIPK3
• Parent Plasmid: pCMV5
• Expressed: HA-HIPK3
• Genbank: NM_005734
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCATGGCCTC
ACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCT
TTTGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAG
GAAAGAAACTATCCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTC
TCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATA
GACCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAA
AACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAGCAAGCTCA
CGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAG
AAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAAT
CATAGCAGCGCAATGCAGATTGTCGATGAATTGTCCATACTTCCTGCAAT
GTTGCAAACCAACATGGGAAATCCAGTGACAGTTGTGACAGCTACCACAG
GATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGTTAGTACAG
CATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTTGATTTTCT
TGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAGAGGGACAA
ATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTTATGCCCGT
CAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACTGAAAATGC
TGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCACCGTAACC
ATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATGACTTTCTG
AAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGGCCCATTCT
TCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGGTTTAATTC
ATGCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTGTTCGGCAG
CCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCATGTATCAAA
GACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGCTCCAGAGA
TTATATTGGGGTTGCCATTTTGTGAAGCCATAGACATGTGGTCATTGGGA
TGTGTGATTGCAGAATTATTTCTTGGATGGCCGCTCTACCCAGGAGCCTT
GGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTTGCCAGGAG
AACAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTTGCAAAGAA
ACAGATATGTCTCATTCTGGTTGGAGATTAAAGACATTGGAAGAGCATGA
GGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACATTTTCAACA
GTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGGAAGGAAGT
GATCTTTTGGCTGAGAAAGCTGATAGAAGAGAATTTGTTAGTCTGTTGAA
GAAAATGTTGCTGATTGATGCAGATTTAAGAATTACTCCAGCTGAGACCC
TGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCCCTCATAGC
AACCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAGTCCCACCT
AAATTCATGTGACACAAATAATCACAACAAAACTTCACTTTTAAGACCAG
TTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTAAAATCGGA
ACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTTGTGCACCA
TGGAATACCTCTGCAGGCAGGAACTGCTCAGTTTGGTTGTGGTGATGCTT
TTCAGCAGACATTGATTATCTGTCCCCCAGCTATTCAAGGTATTCCTGCA
ACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAATACAGTTCC
ACTTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCGACCAGGAG
TTCTTTCTCAGACGTGGTCTGGTAGAACACAGCAGATGCTGGTGCCTGCC
TGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTAACTTCTGA
GAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGATGATTTCAT
GCAGCAATCATTATAACTCAGTGATGCCGCAGCCTCTTCTGACCAATCAG
ATAACTTTATCTGCCCCTCAGCCAGTTAGTGTGGGGATTGCACATGTTGT
CTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCAGAACAGGA
GTAATTCATTACAGAATACCAATATCCCACATTCAGCATTTATTTCTCCA
AAGATAATTAATGGGAAAGATGTCGAGGAAGTAAGTTGTATAGAAACACA
GGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGAAACATCTA
TCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGCAAACCATC
ATTATTGCCGACTCCCCGAGTCCTGCAGTGAGTGTCATCACTATCAGCAG
TGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACTCAGAGAATGTA
AAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATATTGATCGG
ATGTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACCAGCTCCTCAGG
GCAGTCCAGCCCATCCCCCTGCAAGAGACCGAATAGTATGTCAGATGAAG
AGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACATCTGACTCT
TCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAGGACACTCA
TGAAAACACAGAATTGGTATCCTCTGCTGACACAGAAACCAAGCCAGCTG
TCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATGGCTTAAATGCC
GATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTAATAAAAGG
ACGATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGGTACTCGTC
AGCAAAAATTGACATCAGCATTCCAGCAGCAGCATTTGAACTTCAGTCAG
GTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAACTTTGGGCA
CAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAATCCATTCA
CTCTTTCTCATGGAAGTCCCAATCACACAGCAGTGCATGCCCACCTGGCT
GGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCATACCCATCATC
AGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGCCTCTCCGT
GTACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCTCCCATCCC
AGTGGCATAGTTCACCAAGTCCCAGTGGGCTTAAATCCCCGTCTGTTACC
ATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCCACCACATT
CTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGAGTCCAACA
AAACTCAGCCAGTATCCATATATGTGAggtacc

Amino Acid Sequence

MYPYDVPDYAMASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNY
PRTYVNGRNFGNSHPPTKGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAG
ATKVIAAQAQQAHVQAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSA
MQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVL
CSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQI
EVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNK
FSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRV
KVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIA
ELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCKETDMS
HSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLA
EKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVK
SCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRS
QALTTSAHSVVHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGK
PTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQV
TPLAPATTTLTSESVAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLS
APQPVSVGIAHVVWPQPATTKKNKQCQNRSNSLQNTNIPHSAFISPKIIN
GKDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIAD
SPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSL
SSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHD
SPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHM
ANTDSICQPLIKGRSAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHF
GSGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTH
LGGQPTLLPYPSSATLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIV
HQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQ
YPYM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5308
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with NM_005734 CDS: ; t332c (V111A) - present in other mRNAs (AAG25990, CAC13164, AAQ02467). ; Delta nt 2306-2368 (delta aa 770-790) - present in all other ESTs and sequences except NM_005734 and AF004849; ; Estimated MW of expressed protein : 132387 Daltons