cDNA Clone E1a

• Plasmid: pCMV5 E1a-PP-GST
• Parent Plasmid: pCMV5
• Expressed: E1a-GST
• Genbank: P22314/X56976
• Tag: GST

Sequence of Insert

aagcttGCCACCATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCTCCCCTGCCCAGTCCGTGTTGTCCGAAGTGCCCTCGGTGCCAACCAACGGAATGGCCAAGAACGGCAGTGAAGCAGACATAGACGAGGGCCTTTACTCCCGGCAGCTGTATGTGTTGGGCCATGAGGCAATGAAGCGGCTCCAGACATCCAGTGTCCTGGTATCAGGCCTGCGGGGCCTGGGCGTGGAGATCGCTAAGAACATCATCCTTGGTGGGGTCAAGGCTGTTACCCTACATGACCAGGGCACTGCCCAGTGGGCTGATCTTTCCTCCCAGTTCTACCTGCGGGAGGAGGACATCGGTAAAAACCGGGCCGAGGTATCACAGCCCCGCCTCGCTGAGCTCAACAGCTATGTGCCTGTCACTGCCTACACTGGACCCCTCGTTGAGGACTTCCTTAGTGGTTTCCAGGTGGTGGTGCTCACCAACACCCCCCTGGAGGACCAGCTGCGAGTGGGTGAGTTCTGTCACAACCGTGGCATCAAGCTGGTGGTGGCAGACACGCGGGGCCTGTTTGGGCAGCTCTTCTGT
GACTTTGGAGAGGAAATGATCCTCACAGATTCCAATGGGGAGCAGCCACT
CAGTGCTATGGTTTCTATGGTTACCAAGGACAACCCCGGTGTGGTTACCT
GCCTGGATGAGGCCCGACACGGGTTTGAGAGCGGGGACTTTGTCTCCTTT
TCAGAAGTACAGGGCATGGTTGAACTCAACGGAAATCAGCCCATGGAGAT
CAAAGTCCTGGGTCCTTATACCTTTAGCATCTGTGACACCTCCAACTTCT
CCGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCTAAGAAG
ATTAGCTTTAAATCCTTGGTGGCCTCACTGGCAGAACCTGACTTTGTGGT
GACGGACTTCGCCAAGTTTTCTCGCCCTGCCCAGCTGCACATTGGCTTCC
AGGCCCTGCACCAGTTCTGTGCTCAGCATGGCCGGCCACCTCGGCCCCGC
AATGAGGAGGATGCAGCAGAACTGGTAGCCTTAGCACAGGCTGTGAATGC
TCGAGCCCTGCCAGCAGTGCAGCAAAATAACCTGGACGAGGACCTCATCC
GGAAGCTGGCATATGTGGCTGCTGGGGATCTGGCACCCATAAACGCCTTC
ATTGGGGGCCTGGCTGCCCAGGAAGTCATGAAGGCCTGCTCCGGGAAGTT
CATGCCCATCATGCAGTGGCTATACTTTGATGCCCTTGAGTGTCTCCCTG
AGGACAAAGAGGTCCTCACAGAGGACAAGTGCCTCCAGCACCAGAACCGT
TATGACGGGCAAGTGGCTGTGTTTGGCTCAGACCTGCAAGAGAAGCTGGG
CAAGCAGAAGTATTTCCTGGTGGGTGCGGGGGCCATTGGCTGTGAGCTGC
TCAAGAACTTTGCCATGATTGGGCTGGGCTGCGGGGAGGGTGGAGAAATC
ATCGTTACAGACATGGACACCATTGAGAAGTCAAATCTGAATCGACAGTT
TCTTTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCTGCTG
CAGCTGTGCGCCAAATGAATCCACATATCCGGGTGACAAGCCACCAGAAC
CGTGTGGGTCCTGACACGGAGCGCATCTATGATGACGATTTTTTCCAAAA
CCTAGATGGCGTGGCCAATGCCCTGGACAACGTGGATGCCCGCATGTACA
TGGACCGCCGCTGTGTCTACTACCGGAAGCCACTGCTGGAGTCAGGCACA
CTGGGCACCAAAGGCAATGTGCAGGTGGTGATCCCCTTCCTGACAGAGTC
GTACAGTTCCAGCCAGGACCCACCTGAGAAGTCCATCCCCATCTGTACCC
TGAAGAACTTCCCTAATGCCATCGAGCACACCCTGCAGTGGGCTCGGGAT
GAGTTTGAAGGCCTCTTCAAGCAGCCAGCAGAAAATGTCAACCAGTACCT
CACAGACCCCAAGTTTGTGGAGCGAACACTGCGGCTGGCAGGCACTCAGC
CCTTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGCTGCAGCGACCA
CAGACCTGGGCTGACTGCGTGACCTGGGCCTGCCACCACTGGCACACCCA
GTACTCGAACAACATCCGGCAGCTGCTGCACAACTTCCCTCCTGACCAGC
TCACAAGCTCAGGAGCGCCGTTCTGGTCTGGGCCCAAACGCTGTCCACAC
CCGCTCACCTTTGATGTCAACAATCCCCTGCATCTGGACTATGTGATGGC
TGCTGCCAACCTGTTTGCCCAGACCTACGGGCTGACAGGCTCTCAGGACC
GAGCTGCTGTGGCCACATTCCTGCAGTCTGTGCAGGTCCCCGAATTCACC
CCCAAGTCTGGCGTCAAGATCCATGTTTCTGACCAGGAGCTGCAGAGCGC
CAATGCCTCTGTTGATGACAGTCGTCTAGAGGAGCTCAAAGCCACTCTGC
CCAGCCCAGACAAGCTCCCTGGATTCAAGATGTACCCCATTGACTTTGAG
AAGGATGATGACAGCAACTTTCATATGGATTTCATCGTGGCTGCATCCAA
CCTCCGGGCAGAAAACTATGACATTCCTTCTGCAGACCGGCACAAGAGCA
AGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACCACAGCAGCC
GTGGTTGGCCTTGTGTGTCTGGAGCTGTACAAGGTTGTGCAGGGGCACCG
ACAGCTTGACTCCTACAAGAATGGTTTCCTCAACTTGGCCCTGCCTTTCT
TTGGTTTCTCTGAACCCCTTGCCGCACCACGTCACCAGTACTATAACCAA
GAGTGGACATTGTGGGATCGCTTTGAGGTACAAGGGCTGCAGCCTAATGG
TGAGGAGATGACCCTCAAACAGTTCCTCGACTATTTTAAGACAGAGCACA
AATTAGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCCTTC
TTCATGCCAGCTGCCAAGCTCAAGGAACGGTTGGATCAGCCGATGACAGA
GATTGTGAGCCGTGTGTCGAAGCGAAAGCTGGGCCGCCACGTGCGGGCGC
TGGTGCTTGAGCTGTGCTGTAACGACGAGAGCGGCGAGGATGTCGAGGTT
CCCTATGTCCGATACACCATCCGCCTGGAAGTTCTGTTCCAGGGGCCCCT
GATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCA
CTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTAT
GAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTT
GGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACAC
AGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGT
GGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTT
GGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAA
CTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTC
GAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCA
TCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACC
CAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATT
GAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGC
ATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTC
CAAAATCGGATTAGggatcc

Amino Acid Sequence

MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADI
DEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVT
LHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGP
LVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFC
DFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSF
SEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKK
ISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPR
NEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAF
IGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQHQNR
YDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEI
IVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQN
RVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGT
LGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARD
EFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRP
QTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPH
PLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFT
PKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFE
KDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAA
VVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQ
EWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSF
FMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEV
PYVRYTIRLEVLFQGPLMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLY
ERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLG
GCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMF
EDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRI
EAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5299
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 144417 Daltons