cDNA Clone EMAPL

• Plasmid: pGEX6P-1-EMAPL (ELP95)
• Parent Plasmid: pGEX6P1
• Expressed: GST-EMAPL (ELP95)
• Genbank: AK093146
• Tag: GST
• Species: Human

Sequence of Insert

gaattcATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGT
GGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCC
ACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCT
CAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAAC
CTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCT
CTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAG
GCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATC
CCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGA
GACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGG
CGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTG
TTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGAT
AAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGAC
GCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTG
GGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTG
GATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAAT
GAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCT
TCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTC
CACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCT
TACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCC
CTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAG
GGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGG
CAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTC
ATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTG
AGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGT
GGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCA
TTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCA
TTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCA
CACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCC
TCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCAT
GCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTT
CCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGT
TGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGC
AATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGC
CATTGGTTCCCATGACAACGTGATCTACATCTATAGTGTTTCCAGTGATG
GTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATC
ACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGG
GGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGA
AGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTG
CTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCC
GCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCAC
GACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCA
GTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCT
CTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTG
CCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCT
TCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGAC
TTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCC
CTGCACACACTGTTTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMELVKAALAEALRLLR
LQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETEVELKS
SPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGILRPLQP
PQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESRGGKDP
LSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEELPSGPPP
ETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGPGGPGG
GGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPVVHIWDSE
TLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEHMLSVWDCSRG
MKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGVPGNGT
LTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPSDSKTP
GRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQWGPGL
VALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQGFSPVIQG
HTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSIDLKETGLCAD
FHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRYSPDGLHL
AIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNFIMSNS
GDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYVPVRSCQGAE
PHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGAGARHA
LSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGRLFPGL
TSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; ; ; Estimated MW of expressed protein : 122033