cDNA Clone mcm3

• Plasmid: pEGFP-C2-mcm3
• Parent Plasmid: pEGFPC2
• Expressed: GFP-mcm3
• Genbank: NM_002388
• Tag: GFP
• Species: Human

Sequence of Insert

gaattcATGGCGGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTATGAGGAGTTCTACGTA
GGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTAC
CTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAAT
GTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCT
ACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGC
CTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCC
TTGAGACAGAATATGGCCTTTCTGTCTACAAGGATCACCAGACCATCACC
ATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCAGCTCCCCCGCTCTGT
GGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACC
GGGTTCAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGC
TACACCTCTGGGACCTTCAGGACTGTCCTGATTGCCTGTAATGTTAAGCA
GATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGCCAAGA
TCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCC
AAGTCATTGGCCCCAAGTATCCATGGGCATGACTATGTCAAGAAAGCAAT
CCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACCTAGAAAATGGCAGCC
ACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCC
AAGTCTCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCC
CACCACTGGCCGGGGCTCCTCTGGAGTGGGTCTGACGGCTGCTGTCACCA
CAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGGTCCTG
GCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACAT
GGATCGCACAGCCATCCATGAAGTGATGGAGCAGGGTCGAGTGACCATTG
CCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGCTGCAGTGTTTTGGCA
GCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGA
GAACATTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCA
TCATGCTGGATCAGATGGATCCTGAGCAGGATCGGGAGATCTCAGACCAT
GTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGCGATGC
TATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACT
TTAGCCAGGAAGATCAGCAGGACACCCAGATTTATGAGAAGCATGACAAC
CTTCTACATGGGACCAAGAAGAAAAAGGAGAAGATGGTGAGTGCAGCATT
CATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACAC
AGGAGTCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAG
GATAGCATGAGCTCAGACACCGCCAGGACATCTCCAGTTACAGCCCGAAC
ACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCGCATGA
GCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAG
TATGCTTACTTTAAGAAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCG
AAGTGAGGATGAATCAGAGACAGAAGATGAAGAGGAGAAAAGCCAAGAGG
ACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAA
GATGGGGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAAT
GCCTCAAGTACACACTCCAAAGACGGCAGACTCACAGGAGACCAAGGAAT
CCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGGTGGCC
CTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCG
CCTCACAGAATCCATCAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTG
AGATCCAGGCTGCTCTGAGCAAGATGCAGGATGACAATCAGGTCATGGTG
TCTGAGGGCATCATCTTCCTCATCTGAgtcgac

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
EFMAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLI
VNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEF
YVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYC
PATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQT
ITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKK
GGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQ
LAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPS
VAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAM
VLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSV
LAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREIS
DHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKH
DNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLR
SQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVEL
VQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPD
AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFK
VALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQV
MVSEGIIFLI*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU5287
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee