cDNA Clone HIPK3

• Plasmid: pEBG6P-1 HIPK3
• Parent Plasmid: pEBG6P
• Expressed: GST-HIPK3
• Genbank: NM_005734
• Tag: GST
• Species: Human

Sequence of Insert

gcggccgcGATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCAATGCAGATTGTCGATGAATTGTCCATACTT
CCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAGTTGTGACAGC
TACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGT
TAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTT
GATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAG
AGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT
ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACT
GAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCA
CCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATG
ACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGG
CCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGG
TTTAATTCATGCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTG
TTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT
GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGC
TCCAGAGATTATATTGGGGTTGCCATTTTGTGAAGCCATAGACATGTGGT
CATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCCGCTCTACCCA
GGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTT
GCCAGGAGAACAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTT
GCAAAGAAACAGATATGTCTCATTCTGGTTGGAGATTAAAGACATTGGAA
GAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT
TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGG
AAGGAAGTGATCTTTTGGCTGAGAAAGCTGATAGAAGAGAATTTGTTAGT
CTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAATTACTCCAGC
TGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCC
CTCATAGCAACCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAG
TCCCACCTAAATTCATGTGACACAAATAATCACAACAAAACTTCACTTTT
AAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA
AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTT
GTGCACCATGGAATACCTCTGCAGGCAGGAACTGCTCAGTTTGGTTGTGG
TGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCTATTCAAGGTA
TTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAAT
ACAGTTCCACTTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCG
ACCAGGAGTTCTTTCTCAGACGTGGTCTGGTAGAACACAGCAGATGCTGG
TGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA
ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGAT
GATTTCATGCAGCAATCATTATAACTCAGTGATGCCGCAGCCTCTTCTGA
CCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGTGGGGATTGCA
CATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCA
GAACAGGAGTAATTCATTACAGAATACCAATATCCCACATTCAGCATTTA
TTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGAAGTAAGTTGTATA
GAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGA
AACATCTATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGC
AAACCATCATTATTGCCGACTCCCCGAGTCCTGCAGTGAGTGTCATCACT
ATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACTCAG
AGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATA
TTGATCGGATGTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACCAGC
TCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGACCGAATAGTATGTC
AGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACAT
CTGACTCTTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAG
GACACTCATGAAAACACAGAATTGGTATCCTCTGCTGACACAGAAACCAA
GCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATGGCT
TAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTA
ATAAAAGGACGATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGG
TACTCGTCAGCAAAAATTGACATCAGCATTCCAGCAGCAGCATTTGAACT
TCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAAC
TTTGGGCACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAA
TCCATTCACTCTTTCTCATGGAAGTCCCAATCACACAGCAGTGCATGCCC
ACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCATAC
CCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGC
CTCTCCGTGTACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCT
CCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGGCTTAAATCCCCGT
CTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCC
ACCACATTCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGA
GTCCAACAAAACTCAGCCAGTATCCATATATGTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTIDAAAMASQ
VLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSH
PPTKGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHV
QAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSAMQIVDELSILPAML
QTNMGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLG
RGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENAD
EYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPILQ
QVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKT
VCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALE
YDQIRYISQTQGLPGEQLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEA
ETGMKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLAEKADRREFVSLLKK
MLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLN
SCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSVVHHG
IPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPL
VTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSES
VAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVW
PQPATTKKNKQCQNRSNSLQNTNIPHSAFISPKIINGKDVEEVSCIETQD
NQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIADSPSPAVSVITISSD
TDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQ
SSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHE
NTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGR
SAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHR
RQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSA
TLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPS
PTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5279
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express HIPK3 with N-terminal GST (contains preScission protease site); N.B. Sequence differences compared with NM_005734 CDS: ; t332c (V111A) - present in other mRNAs (AAG25990, CAC13164, AAQ02467). ; Delta nt 2306-2368 (delta aa 770-790) - present in all other ESTs and sequences except NM_005734 and AF004849; ; Estimated MW of expressed protein : 159376 Daltons