cDNA Clone DNA-PK

• Plasmid: pGEX6P-1-DNA-PK CAT
• Parent Plasmid: pGEX6P1
• Expressed: GST-DNA-PK CAT
• Genbank: NP_008835
• Tag: GST
• Species: Human

Sequence of Insert

gaattcAATAAAAAAAACATTGAAAAAATGTATGAAAGAATGTATGCAGCCTTGGGTGACCCAAAGGCTCCAGGCCTGGGGGCCTTTAGAAGGAAGTTTATTCAGACTTTTGGAAAAGAATTTGATAAACATTTTGGGAAAGGAGGTTCTAAACTACTGAGAATGAAGCTCAGTGACTTCAACGACATTACCAACATGCTACTTTTAAAAATGAACAAAGACTCAAAGCCCCCTGGGAATCTGAAAGAATGTTCACCCTGGATGAGCGACTTCAAAGTGGAGTTCCTGAGAAATGAGCTGGAGATT
CCCGGTCAGTATGACGGTAGGGGAAAGCCATTGCCAGAGTACCACGTGCG
AATCGCCGGGTTTGATGAGCGGGTGACAGTCATGGCGTCTCTGCGAAGGC
CCAAGCGCATCATCATCCGTGGCCATGACGAGAGGGAACACCCTTTCCTG
GTGAAGGGTGGCGAGGACCTGCGGCAGGACCAGCGCGTGGAGCAGCTCTT
CCAGGTCATGAATGGGATCCTGGCCCAAGACTCCGCCTGCAGCCAGAGGG
CCCTGCAGCTGAGGACCTATAGCGTTGTGCCCATGACCTCCAGGTTAGGA
TTAATTGAGTGGCTTGAAAATACTGTTACCTTGAAGGACCTTCTTTTGAA
CACCATGTCCCAAGAGGAGAAGGCGGCTTACCTGAGTGATCCCAGGGCAC
CGCCGTGTGAATATAAAGATTGGCTGACAAAAATGTCAGGAAAACATGAT
GTTGGAGCTTACATGCTAATGTATAAGGGCGCTAATCGTACTGAAACAGT
CACGTCTTTTAGAAAACGAGAAAGTAAAGTGCCTGCTGATCTCTTAAAGC
GGGCCTTCGTGAGGATGAGTACAAGCCCTGAGGCTTTCCTGGCGCTCCGC
TCCCACTTCGCCAGCTCTCACGCTCTGATATGCATCAGCCACTGGATCCT
CGGGATTGGAGACAGACATCTGAACAACTTTATGGTGGCCATGGAGACTG
GCGGCGTGATCGGGATCGACTTTGGGCATGCGTTTGGATCCGCTACACAG
TTTCTGCCAGTCCCTGAGTTGATGCCTTTTCGGCTAACTCGCCAGTTTAT
CAATCTGATGTTACCAATGAAAGAAACGGGCCTTATGTACAGCATCATGG
TACACGCACTCCGGGCCTTCCGCTCAGACCCTGGCCTGCTCACCAACACC
ATGGATGTGTTTGTCAAGGAGCCCTCCTTTGATTGGAAAAATTTTGAACA
GAAAATGCTGAAAAAAGGAGGGTCATGGATTCAAGAAATAAATGTTGCTG
AAAAAAATTGGTACCCCCGACAGAAAATATGTTACGCTAAGAGAAAGTTA
GCAGGTGCCAATCCAGCAGTCATTACTTGTGATGAGCTACTCCTGGGTCA
TGAGAAGGCCCCTGCCTTCAGAGACTATGTGGCTGTGGCACGAGGAAGCA
AAGATCACAACATTCGTGCCCAAGAACCAGAGAGTGGGCTTTCAGAAGAG
ACTCAAGTGAAGTGCCTGATGGACCAGGCAACAGACCCCAACATCCTTGG
CAGAACCTGGGAAGGATGGGAGCCCTGGATGTGAgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFNKKNIEKMYERMYAAL
GDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLL
LKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHV
RIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQL
FQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLL
NTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTET
VTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWI
LGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQF
INLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFE
QKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLG
HEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNIL
GRTWEGWEPWM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5276
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 87392 Daltons