cDNA Clone HIPK3

• Plasmid: pFastBac.HTb HIPK3
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-HIPK3
• Genbank: NM_005734
• Tag: 6His
• Species: Human

Sequence of Insert

gcggccgcGATGGCCTCACAAGTCTTGGTCTACCCACCATATGTTTATCAAACTCAGTCAAGTGCCTTTTGTAGTGTGAAGAAACTCAAAGTAGAGCCAAGCAGTTGTGTATTCCAGGAAAGAAACTATCCACGGACCTATGTGAATGGTAGAAACTTTGGAAATTCTCATCCTCCCACTAAGGGTAGTGCTTTTCAGACAAAGATACCATTTAATAGACCTCGAGGACACAACTTTTCATTGCAGACAAGTGCTGTTGTTTTGAAAAACACTGCAGGTGCTACAAAGGTCATAGCAGCTCAGGCACAGCAAGCTCACGTGCAGGCACCTCAGATTGGGGCGTGGCGAAACAGATTGCATTTCCTAGAAGGCCCCCAGCGATGTGGATTGAAGCGCAAGAGTGAGGAGTTGGATAATCATAGCAGCGCAATGCAGATTGTCGATGAATTGTCCATACTT
CCTGCAATGTTGCAAACCAACATGGGAAATCCAGTGACAGTTGTGACAGC
TACCACAGGATCAAAACAGAATTGTACCACTGGAGAAGGTGACTATCAGT
TAGTACAGCATGAAGTCTTATGCTCCATGAAAAATACTTACGAAGTCCTT
GATTTTCTTGGTCGAGGCACGTTTGGCCAGGTAGTTAAATGCTGGAAAAG
AGGGACAAATGAAATTGTAGCAATCAAAATTTTGAAGAATCATCCTTCTT
ATGCCCGTCAAGGTCAAATAGAAGTGAGCATATTAGCAAGGCTCAGTACT
GAAAATGCTGATGAATATAACTTTGTACGAGCTTATGAATGCTTTCAGCA
CCGTAACCATACTTGTTTAGTCTTTGAGATGCTGGAACAAAACTTGTATG
ACTTTCTGAAACAAAATAAATTTAGTCCCCTGCCACTAAAAGTGATTCGG
CCCATTCTTCAACAAGTGGCCACTGCACTGAAAAAATTGAAAAGTCTTGG
TTTAATTCATGCTGATCTCAAGCCAGAGAATATTATGTTGGTGGATCCTG
TTCGGCAGCCTTACAGGGTTAAAGTAATAGACTTTGGGTCGGCCAGTCAT
GTATCAAAGACTGTTTGTTCAACATATCTACAATCTCGGTACTACAGAGC
TCCAGAGATTATATTGGGGTTGCCATTTTGTGAAGCCATAGACATGTGGT
CATTGGGATGTGTGATTGCAGAATTATTTCTTGGATGGCCGCTCTACCCA
GGAGCCTTGGAGTATGATCAGATTCGATACATTTCTCAGACTCAAGGTTT
GCCAGGAGAACAGTTGTTAAATGTGGGTACTAAATCCACAAGATTTTTTT
GCAAAGAAACAGATATGTCTCATTCTGGTTGGAGATTAAAGACATTGGAA
GAGCATGAGGCAGAGACAGGAATGAAGTCTAAAGAAGCCAGAAAATACAT
TTTCAACAGTCTGGATGATGTAGCGCATGTGAACACAGTGATGGATTTGG
AAGGAAGTGATCTTTTGGCTGAGAAAGCTGATAGAAGAGAATTTGTTAGT
CTGTTGAAGAAAATGTTGCTGATTGATGCAGATTTAAGAATTACTCCAGC
TGAGACCCTGAACCATCCTTTTGTTAATATGAAACATCTTCTAGATTTCC
CTCATAGCAACCATGTAAAGTCCTGTTTTCATATTATGGATATTTGTAAG
TCCCACCTAAATTCATGTGACACAAATAATCACAACAAAACTTCACTTTT
AAGACCAGTTGCTTCAAGCAGTACTGCTACACTGACTGCAAATTTTACTA
AAATCGGAACATTAAGAAGTCAGGCATTGACCACATCTGCTCATTCAGTT
GTGCACCATGGAATACCTCTGCAGGCAGGAACTGCTCAGTTTGGTTGTGG
TGATGCTTTTCAGCAGACATTGATTATCTGTCCCCCAGCTATTCAAGGTA
TTCCTGCAACACATGGTAAACCCACCAGTTATTCAATAAGGGTAGATAAT
ACAGTTCCACTTGTAACTCAGGCCCCAGCTGTGCAGCCACTACAGATCCG
ACCAGGAGTTCTTTCTCAGACGTGGTCTGGTAGAACACAGCAGATGCTGG
TGCCTGCCTGGCAACAGGTGACACCCCTGGCTCCTGCTACTACTACACTA
ACTTCTGAGAGTGTGGCTGGTTCACACAGGCTTGGAGACTGGGGGAAGAT
GATTTCATGCAGCAATCATTATAACTCAGTGATGCCGCAGCCTCTTCTGA
CCAATCAGATAACTTTATCTGCCCCTCAGCCAGTTAGTGTGGGGATTGCA
CATGTTGTCTGGCCTCAGCCTGCCACTACCAAGAAAAATAAACAGTGCCA
GAACAGGAGTAATTCATTACAGAATACCAATATCCCACATTCAGCATTTA
TTTCTCCAAAGATAATTAATGGGAAAGATGTCGAGGAAGTAAGTTGTATA
GAAACACAGGACAATCAGAACTCAGAAGGAGAGGCAAGAAATTGCTGTGA
AACATCTATCAGACAGGACTCTGATTCATCAGTTTCAGACAAACAGCGGC
AAACCATCATTATTGCCGACTCCCCGAGTCCTGCAGTGAGTGTCATCACT
ATCAGCAGTGACACTGATGAGGAAGAGACTTCCCAGAGACATTCACTCAG
AGAATGTAAAGGTAGTCTAGATTGTGAAGCTTGCCAGAGCACTTTGAATA
TTGATCGGATGTGTTCATTAAGTAGTCCTGATAGTACTCTGAGTACCAGC
TCCTCAGGGCAGTCCAGCCCATCCCCCTGCAAGAGACCGAATAGTATGTC
AGATGAAGAGCAAGAAAGTAGTTGTGATACGGTGGATGGCTCTCCGACAT
CTGACTCTTCCGGGCATGACAGTCCATTTGCAGAGAGCACTTTTGTGGAG
GACACTCATGAAAACACAGAATTGGTATCCTCTGCTGACACAGAAACCAA
GCCAGCTGTCTGTTCTGTTGTGGTGCCACCAGTGGAACTAGAAAATGGCT
TAAATGCCGATGAGCATATGGCAAACACAGATTCTATATGCCAGCCATTA
ATAAAAGGACGATCTGCCCCTGGAAGATTAAACCAGCCTTCTGCAGTGGG
TACTCGTCAGCAAAAATTGACATCAGCATTCCAGCAGCAGCATTTGAACT
TCAGTCAGGTTCAGCACTTTGGATCTGGGCATCAAGAGTGGAATGGAAAC
TTTGGGCACAGAAGACAGCAAGCTTATATTCCTACTAGTGTTACCAGTAA
TCCATTCACTCTTTCTCATGGAAGTCCCAATCACACAGCAGTGCATGCCC
ACCTGGCTGGAAATACACACCTCGGAGGACAGCCTACTCTACTTCCATAC
CCATCATCAGCCACCCTCAGTAGTGCTGCACCAGTGGCCCACCTGTTAGC
CTCTCCGTGTACCTCAAGACCTATGTTACAGCATCCAACTTATAATATCT
CCCATCCCAGTGGCATAGTTCACCAAGTCCCAGTGGGCTTAAATCCCCGT
CTGTTACCATCCCCAACCATTCATCAGACTCAGTACAAACCAATCTTCCC
ACCACATTCTTACATTGCAGCATCACCTGCATATACTGGATTTCCACTGA
GTCCAACAAAACTCAGCCAGTATCCATATATGTGAgcggccgc

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGIQRPTSTSSLVAAAMASQVLV
YPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPT
KGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAP
QIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTN
MGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLGRGT
FGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYN
FVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPILQQVA
TALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCS
TYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQ
IRYISQTQGLPGEQLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETG
MKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLAEKADRREFVSLLKKMLL
IDADLRITPAETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLNSCD
TNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSVVHHGIPL
QAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPLVTQ
APAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSESVAG
SHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQP
ATTKKNKQCQNRSNSLQNTNIPHSAFISPKIINGKDVEEVSCIETQDNQN
SEGEARNCCETSIRQDSDSSVSDKQRQTIIIADSPSPAVSVITISSDTDE
EETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSP
SPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTE
LVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAP
GRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHRRQQ
AYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSATLS
SAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPSPTI
HQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU5275
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression plasmid with N terminal 6His tag; Sequence differences compared with NM_005734 CDS: ; t332c (V111A) - present in other mRNAs (AAG25990, CAC13164, AAQ02467). ; Delta nt 2306-2368 (delta aa 770-790) - present in all other ESTs and sequences except NM_005734 and AF004849; ; Estimated MW of expressed protein : 135982 Daltons