cDNA Clone DNA-PK

• Plasmid: pEBG6P-1 DNA-PK CAT
• Parent Plasmid: pEBG6P
• Expressed: GST-DNA-PK CAT
• Genbank: NP_008835
• Tag: GST
• Species: Human

Sequence of Insert

actagtAATAAAAAAAACATTGAAAAAATGTATGAAAGAATGTATGCAGCCTTGGGTGACCCAAAGGCTCCAGGCCTGGGGGCCTTTAGAAGGAAGTTTATTCAGACTTTTGGAAAAGAATTTGATAAACATTTTGGGAAAGGAGGTTCTAAACTACTGAGAATGAAGCTCAGTGACTTCAACGACATTACCAACATGCTACTTTTAAAAATGAACAAAGACTCAAAGCCCCCTGGGAATCTGAAAGAATGTTCACCCTGGATGAGCGACTTCAAAGTGGAGTTCCTGAGAAATGAGCTGGAGATT
CCCGGTCAGTATGACGGTAGGGGAAAGCCATTGCCAGAGTACCACGTGCG
AATCGCCGGGTTTGATGAGCGGGTGACAGTCATGGCGTCTCTGCGAAGGC
CCAAGCGCATCATCATCCGTGGCCATGACGAGAGGGAACACCCTTTCCTG
GTGAAGGGTGGCGAGGACCTGCGGCAGGACCAGCGCGTGGAGCAGCTCTT
CCAGGTCATGAATGGGATCCTGGCCCAAGACTCCGCCTGCAGCCAGAGGG
CCCTGCAGCTGAGGACCTATAGCGTTGTGCCCATGACCTCCAGGTTAGGA
TTAATTGAGTGGCTTGAAAATACTGTTACCTTGAAGGACCTTCTTTTGAA
CACCATGTCCCAAGAGGAGAAGGCGGCTTACCTGAGTGATCCCAGGGCAC
CGCCGTGTGAATATAAAGATTGGCTGACAAAAATGTCAGGAAAACATGAT
GTTGGAGCTTACATGCTAATGTATAAGGGCGCTAATCGTACTGAAACAGT
CACGTCTTTTAGAAAACGAGAAAGTAAAGTGCCTGCTGATCTCTTAAAGC
GGGCCTTCGTGAGGATGAGTACAAGCCCTGAGGCTTTCCTGGCGCTCCGC
TCCCACTTCGCCAGCTCTCACGCTCTGATATGCATCAGCCACTGGATCCT
CGGGATTGGAGACAGACATCTGAACAACTTTATGGTGGCCATGGAGACTG
GCGGCGTGATCGGGATCGACTTTGGGCATGCGTTTGGATCCGCTACACAG
TTTCTGCCAGTCCCTGAGTTGATGCCTTTTCGGCTAACTCGCCAGTTTAT
CAATCTGATGTTACCAATGAAAGAAACGGGCCTTATGTACAGCATCATGG
TACACGCACTCCGGGCCTTCCGCTCAGACCCTGGCCTGCTCACCAACACC
ATGGATGTGTTTGTCAAGGAGCCCTCCTTTGATTGGAAAAATTTTGAACA
GAAAATGCTGAAAAAAGGAGGGTCATGGATTCAAGAAATAAATGTTGCTG
AAAAAAATTGGTACCCCCGACAGAAAATATGTTACGCTAAGAGAAAGTTA
GCAGGTGCCAATCCAGCAGTCATTACTTGTGATGAGCTACTCCTGGGTCA
TGAGAAGGCCCCTGCCTTCAGAGACTATGTGGCTGTGGCACGAGGAAGCA
AAGATCACAACATTCGTGCCCAAGAACCAGAGAGTGGGCTTTCAGAAGAG
ACTCAAGTGAAGTGCCTGATGGACCAGGCAACAGACCCCAACATCCTTGG
CAGAACCTGGGAAGGATGGGAGCCCTGGATGTGAactagt

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSNKKNIEKMYER
MYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDI
TNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPL
PEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQ
RVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTL
KDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGA
NRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALIC
ISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFR
LTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFD
WKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCD
ELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQAT
DPNILGRTWEGWEPWM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5257
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express DNA-PK 3602-4128(end) with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 87816 Daltons