cDNA Clone EMAPL

• Plasmid: pEBG6P-1 EMAPL (ELP95)
• Parent Plasmid: pEBG6P
• Expressed: GST-EMAPL (ELP95)
• Genbank: AK093146
• Tag: GST
• Species: Human

Sequence of Insert

actagtATGGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGT
GGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCC
ACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCT
CAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAAC
CTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCT
CTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAG
GCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATC
CCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGA
GACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCT
CTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGT
GTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGG
CGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTG
TTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGAT
AAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGAC
GCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTG
GGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTG
GATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAAT
GAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCT
TCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTC
CACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCT
TACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCC
CTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAG
GGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGG
CAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTC
ATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTG
AGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGT
GGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCA
TTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCA
TTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCA
CACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCC
TCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCAT
GCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTT
CCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGT
TGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGC
AATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGC
CATTGGTTCCCATGACAACGTGATCTACATCTATAGTGTTTCCAGTGATG
GTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATC
ACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGG
GGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGA
AGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTG
CTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCC
GCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCAC
GACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCA
GTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCT
CTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTG
CCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCT
TCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGAC
TTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCC
CTGCACACACTGTTTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMELVKAALAEA
LRLLRLQVPPSSLQGSGTPAPPGDSLAAPPGLPPTCTPSLVSRGTQTETE
VELKSSPGPPGLSNGPPAPQGASEEPSGTQSEGGGSSSSGAGSPGPPGIL
RPLQPPQRADTPRRNSSSSSSPSERPRQKLSRKAISSANLLVRSGSTESR
GGKDPLSSPGGPGSRRSNYNLEGISVKMFLRGRPITMYIPSGIRSLEELP
SGPPPETLSLDWVYGYRGRDSRSNLFVLRSGEVVYFIACVVVLYRPGGGP
GGPGGGGQRHYRGHTDCVRCLAVHPDGVRVASGQTAGVDKDGKPLQPVVH
IWDSETLLKLQEIGLGAFERGVGALAFSAADQGAFLCVVDDSNEHMLSVW
DCSRGMKLAEIKSTNDSVLAVGFNPRDSSCIVTSGKSHVHFWNWSGGVGV
PGNGTLTRKQGVFGKYKKPKFIPCFVFLPDGDILTGDSEGNILTWGRSPS
DSKTPGRGGAKETYGIVAQAHAHEGSIFALCLRRDGTVLSGGGRDRRLVQ
WGPGLVALQEAEIPEHFGAVRAIAEGLGSELLVGTTKNALLRGDLAQGFS
PVIQGHTDELWGLCTHPSQNRFLTCGHDRQLCLWDGESHALAWSIDLKET
GLCADFHPSGAVVAVGLNTGRWLVLDTETREIVSDVIDGNEQLSVVRYSP
DGLHLAIGSHDNVIYIYSVSSDGAKSSRFGRCMGHSSFITHLDWSKDGNF
IMSNSGDYEILYWDVAGGCKQLKNRYESRDREWATYTCVLGFHVYVPVRS
CQGAEPHVRGPRQPRDQRPIHARRLAPRLAGRQGRQHLPVASAGRWGRGA
GARHALSNPLPVPRLLPRRLIAAWRDRLARRRGPAPPCPSLAQSPTTRGR
LFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5243
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express EMAPL with N-terminal GST (contains preScission protease site); ; N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 122457 Daltons