cDNA Clone EMAPL

• Plasmid: pCMV5-HA EMAPL (ELP95)
• Parent Plasmid: pCMV5
• Expressed: HA-EMAPL (ELP95)
• Genbank: AK093146
• Tag: HA
• Species: Human

Sequence of Insert

gaattcGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAACTGGTAAAGGCAGCCCTGGCAGAAGCCCTTCGCCTGCTGCGGCTGCAGGTGCCCCCTTCCTCCCTGCAGGGCTCTGGCACACCAGCTCCTCCGGGGGACAGTCTTGCAGCCCCCCCAGGACTGCCACCCACGTGCACCCCTTCCTTGGTGAGCCGAGGCACCCAGACGGAGACAGAGGTGGAGCTCAAGTCATCCCCTGGACCCCCTGGCCTGAGCAATGGACCCCCAGCCCCTCAGGGGGCCAGCGAAGAGCCTAGCGGGACCCAATCTGAAGGAGGGGGCAGCAGCAGCAGTGGTGCTGGCTCCCCTGGCCCCCCGGGGATCCTCAGGCCCTTGCAGCCCCCACAGCGTGCTGACACGCCGCGAAGAAATTCTTCCTCCTCCTCATCCCCCTCAGAGCGGCCTCGGCAGAAGCTCTCCAGGAAGGCAATCTCCTCCGCCAACCTGTTAGTGCGGTCCGGGAGCACAGAGAGCCGTGGGGGAAAAGACCCCCTCTCCAGCCCTGGGGGCCCTGGATCTCGGAGGAGCAATTACAATTTGGAAGGCATCTCAGTGAAGATGTTCCTTCGAGGGCGCCCCATTACCATGTACATCCCGTCTGGCATCCGCAGCCTTGAGGAGCTGCCGAGTGGCCCACCGCCAGAGACCCTCAGCCTTGACTGGGTTTATGGGTACAGGGGTCGTGACTCCCGCTCTAATCTGTTTGTGTTGCGCTCTGGGGAGGTGGTCTACTTTATCGCCTGTGTGGTGGTGCTGTACCGGCCTGGAGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACATTACCGGGGGCACACAGACTGCGTTCGATGCCTTGCTGTTCACCCTGATGGTGTTCGGGTAGCCTCGGGACAGACAGCTGGAGTGGATAAGGATGGAAAGCCCCTGCAGCCTGTGGTTCACATCTGGGACTCAGAGACGCTGTTGAAACTGCAGGAGATTGGACTGGGGGCCTTCGAGCGGGGTGTTGGGGCCCTGGCCTTTTCAGCTGCGGATCAGGGTGCCTTTCTTTGTGTGGTGGATGATTCCAATGAGCACATGCTGTCGGTGTGGGACTGCAGCCGGGGAATGAAGCTGGCTGAGATCAAGAGTACAAATGACTCAGTCTTGGCCGTTGGCTTCAACCCTCGTGACAGCAGCTGCATCGTCACCAGTGGGAAATCTCACGTCCACTTCTGGAATTGGAGTGGTGGAGTAGGGGTTCCTGGGAATGGGACCCTTACCCGGAAACAGGGTGTCTTTGGGAAATACAAGAAACCCAAGTTTATCCCTTGCTTTGTGTTCCTTCCGGATGGAGACATTCTCACCGGAGACTCAGAGGGGAACATTCTCACCTGGGGGCGGAGCCCTTCAGATTCCAAGACCCCAGGCAGGGGTGGCGCCAAAGAGACCTATGGGATTGTGGCCCAGGCTCACGCTCATGAAGGTTCTATCTTCGCCTTGTGTCTCCGGAGGGACGGGACAGTGCTGAGTGGTGGCGGGCGGGACCGCCGGCTGGTACAGTGGGGGCCCGGGTTGGTGGCCCTCCAGGAGGCTGAGATTCCCGAGCACTTCGGGGCCGTGCGAGCCATTGCTGAAGGGCTTGGCTCTGAGCTGCTGGTGGGAACCACGAAGAATGCATTGCTGAGGGGAGACCTGGCCCAGGGCTTCTCCCCTGTAATCCAGGGCCACACTGATGAGCTCTGGGGGCTCTGCACACACCCCTCCCAGAACCGCTTCCTCACCTGCGGCCACGACCGGCAGCTCTGCCTGTGGGATGGGGAGAGCCATGCACTGGCCTGGAGCATCGACCTCAAGGAGACTGGTCTCTGTGCTGACTTCCACCCGAGTGGGGCAGTTGTGGCCGTAGGACTGAACACGGGGAGGTGGTTGGTTTTGGACACAGAGACCAGAGAGATCGTGTCTGATGTCATTGATGGC
AATGAGCAGCTCTCAGTGGTCCGGTACAGCCCAGATGGGTTGCACCTGGC
CATTGGTTCCCATGACAACGTGATCTACATCTATAGTGTTTCCAGTGATG
GTGCCAAATCCAGCCGCTTTGGCCGCTGTATGGGTCACTCCAGCTTCATC
ACTCATCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGG
GGACTATGAGATTCTTTACTGGGACGTGGCTGGAGGCTGCAAGCAGCTGA
AGAATCGCTATGAGAGCCGAGACCGGGAATGGGCTACCTACACCTGTGTG
CTGGGCTTTCACGTCTACGTACCCGTGCGCTCGTGCCAAGGCGCCGAGCC
GCATGTACGGGGGCCACGGCAGCCACGTGACCAGCGTCCGATTCACGCAC
GACGACTCGCACCTCGTCTCGCTGGGCGGCAAGGACGCCAGCATCTTCCA
GTGGCGAGTGCTGGGCGCTGGGGGCGCGGGGCCGGCGCCCGCCACGCCCT
CTCGAACCCCCTCCCTGTCCCCCGCCTCCTCCCTCGACGTTTGATCGCTG
CCTGGCGGGACCGACTGGCCCGGCGGCGTGGCCCCGCCCCGCCCTGCCCT
TCCCTGGCCCAATCCCCCACGACTAGGGGCCGACTCTTTCCTGGACTGAC
TTCGAGACATTCCCGATCGCGCATTTTCCTGGAGGGCGCGAACGGCGCCC
CTGCACACACTGTTTAGgtcgac

Amino Acid Sequence

MYPYDVPDYAELVKAALAEALRLLRLQVPPSSLQGSGTPAPPGDSLAAPP
GLPPTCTPSLVSRGTQTETEVELKSSPGPPGLSNGPPAPQGASEEPSGTQ
SEGGGSSSSGAGSPGPPGILRPLQPPQRADTPRRNSSSSSSPSERPRQKL
SRKAISSANLLVRSGSTESRGGKDPLSSPGGPGSRRSNYNLEGISVKMFL
RGRPITMYIPSGIRSLEELPSGPPPETLSLDWVYGYRGRDSRSNLFVLRS
GEVVYFIACVVVLYRPGGGPGGPGGGGQRHYRGHTDCVRCLAVHPDGVRV
ASGQTAGVDKDGKPLQPVVHIWDSETLLKLQEIGLGAFERGVGALAFSAA
DQGAFLCVVDDSNEHMLSVWDCSRGMKLAEIKSTNDSVLAVGFNPRDSSC
IVTSGKSHVHFWNWSGGVGVPGNGTLTRKQGVFGKYKKPKFIPCFVFLPD
GDILTGDSEGNILTWGRSPSDSKTPGRGGAKETYGIVAQAHAHEGSIFAL
CLRRDGTVLSGGGRDRRLVQWGPGLVALQEAEIPEHFGAVRAIAEGLGSE
LLVGTTKNALLRGDLAQGFSPVIQGHTDELWGLCTHPSQNRFLTCGHDRQ
LCLWDGESHALAWSIDLKETGLCADFHPSGAVVAVGLNTGRWLVLDTETR
EIVSDVIDGNEQLSVVRYSPDGLHLAIGSHDNVIYIYSVSSDGAKSSRFG
RCMGHSSFITHLDWSKDGNFIMSNSGDYEILYWDVAGGCKQLKNRYESRD
REWATYTCVLGFHVYVPVRSCQGAEPHVRGPRQPRDQRPIHARRLAPRLA
GRQGRQHLPVASAGRWGRGAGARHALSNPLPVPRLLPRRLIAAWRDRLAR
RRGPAPPCPSLAQSPTTRGRLFPGLTSRHSRSRIFLEGANGAPAHTV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5242
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Sequence differences compared with AK093146 CDS:; delta cag 108-110 (delta S36)*, ; c867t (silent G289)*, ; a1057g (N353D)*, ; c1141t (L381)*, ; t1996c (Y666H), ; t1341c (silent T447) ; ; * present in other published mRNA sequences for EMAPL, e.g. XP_508493, BC075824, BC050467, AL833388; ; Estimated MW of expressed protein : 95936 Daltons