cDNA Clone YBCP1

• Plasmid: pEBG6P-1 YBCP1
• Parent Plasmid: pEBG6P
• Expressed: GST-YBCP1
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAAGATGGTACCCCAAAGCATATCATCCAGATGACAGGATTTAAGATGGAAGAAAAAGAAGCGCTAGTCAAATTACTTTTAAAACTAGATTGCACTTTTATTAAGAGTGAGAAATACAAAAATTGTACACATCTTATAGCTGAACGCCTATGTAAGAGTGAAAAATTTTTAGCAGCTTGTGCGGCAGGAAAGTGGATACTAACCAAGGACTATATAATTCATAGTGCCAAAAGTGGCAGATGGCTTGATGAAACAACTTATGAATGGGGATATAAAATTGAAAAAGATTCCCGTTAT
TCACCTCAAATGCAATCTGCACCTAAAAGATGGCGTGAAGAACTGAAACG
CACTGGTGCTCCAGGAGCCTTCCACAGATGGAAAGTTGTCCTCCTTGTTA
GAACTGATAAGCGAAGCGATTCTCTTATAAGAGTTTTGGAGGCTGGAAAG
GCAAATGTTATTTTACCAAAAAGTTCACCAAGTGGAATAACTCATGTGAT
TGCCAGTAATGCAAGAATTAAAGCTGAGAAAGAAAAAGATAACTTTAAGG
CTCCATTTTATCCAATTCAGTATCTAGGGGATTTTCTTTTAGAGAAAGAA
ATTCAGAATGATGAAGATTCCCAAACCAATTCTGTTTGGACTGAACATAG
CAATGAAGAAACAAACAAAGATTTCAGGAAAGATGCAGGATTTCTTGAAA
TGAAAGGTGCCTTAAGAGAGACCATGTATAGAACCCAGAAAGAAATGCAA
AATCATGAAGATGTTAATGTTGGTTCTATTTTGATTCAACATCACAAAAA
AGAAAAATTCAGTGGTTCCAGTAAAGATTTGAAATTTGTTAAAATGAGAA
ATACCTTTGGAAGCCATACATATGAAAATCAGAAGGAAATTAAGAAAAAA
GATGAAGATATTCAGAGGAGTTATACTTTGAGGAGAAAACGCAAGAAAGG
AAAAGAAAGCAATTGCAAGAAAGGCGTTGAACATGAAAAAATAAAAAGTA
CCTTAAGAAGGCACATATATAATAGAGATCAGAAAGAAATGAAGAATTCT
ATTTTTGCTGAATATGCCAAAGAATCAAAAGCCATGGCTATTAAGACAGA
TGTGGATGTTGTTGAAATAAAAAATACCTTAAGGAAGCACATATATAGAG
CTCAGGCTGTCAGATACAACTGCATTAGAATAGATAAACAACCAGTGTAC
AACGTAGAGGTTAAAAATGCTGAATTTCCCAGAGGTGTATTAAATTTAAT
TGAAAGCCTCATAGAAGGACATTTTTTTAAAGAAGCAATTGAGGAACTTT
CCACTTTGCAGGCACATTATATCCCTCCTGTATGCGTTCTTCATGCCCTT
CTAGAGAACGTTCTACAGGATAACATAGATACATTTTCTGGTCGATACTT
TCATATATTGTCAGCTCTTCTTCACCTGCATCCTCCTTGGAAGTCTCCAG
CCATGTCGAGATATTATTTAGAGTTGTTTCAGTGTCCAACTTGTATGAAA
GGAGCATGGTCTTTAGTAGAAGTCCTTATCAGGTCTTGCCTTTTCAATGA
AAGCTTTTGTCATCAAATTTCAGAAAATATTGGCTCCAAGGTGCTCCACC
TGACGCTACTCAAATTTTTCTTTAATTTAATTGAAAGTGAAGTACAACAT
CTGAGTCAAAAGTTGTATGACTGGTCAGATTCTCAGAATCTGAAAATAAC
AGGAAAGGCAATGCTTCTTGAAATTTTTTGGTCAGGAAGTGAAACCTCTG
GGCTTTTGACCAAACCAGTAAATATGCTTTTGGAATGGACTATATATTCT
CACAAGGAAAAATTCAAGTCTAATGATGTCTTCAAACATGAACTAGCTTA
CTTATTGGCTGGAATTCTTGGAGCAGCAATAGATTATTGGATTTTCCTTG
GTCTTAAGATGGGTAGAAATGTGATGCGACACATGTCTGATGACTTAGGA
AGTTATGTTTCTCTTTCGTGTGATGACTTTTCTTCACAGGAATTAGAGAT
TTTCATTTGCTCCTTTTCCTCCTCCTGGCTTCAAATGTTTGTTGCAGAGG
CAGTCTTTAAAAAGTTGTGTCTACAGAGCTCTGGCAGTGTTTCTTCTGAG
CCACTCTCTCTTCAGAAAATGGTATATTCCTATTTATCAGCCTTGGGGAA
AACTGGTGTGCTTGGGTCTGGAAAGATTCAGGTGTCAAAGAAAATAGGAC
AGCGGCCTTGTTTTGACTCTCAGAGAACCTTACTAATGCTGAATGGTACT
AAACAAAAACAAGTCGAAGGGCTGCCAGAGTTACTAGACCTGAACCTTGC
TAAATGTTCCTCATCATTAAAAAAATTGAAAAAGAAGTCAGAAGGAGAAT
TGTCATGTTCCAAGGAGAATTGCCCCTCTGTAGTTAAAAAGATGAATTTT
CACAAGACTAATCTAAAAGGAGAAACAGCCCTGCATAGAGCTTGCATAAA
TAACCAAGTGGAGAAATTGATTCTTCTTCTCTCTTTGCCAGGAATAGACA
TCAATGTTAAAGACAATGCTGGCTGGACGCCTTTGCATGAAGCCTGTAAC
TATGGCAACACAGTGTGTGTCCAGGAAATTTTGCAACGTTGTCCAGAGGT
AGATCTGCTCACTCAAGTGGACGGGGTGACTCCTTTGCATGATGCACTGT
CAAACGGACATGTAGAAATTGGCAAGCTGCTACTACAGCATGGGGGCCCA
GTGCTTTTACAACAGAGGAATGCTAAGGGAGAATTGCCCTTGGATTATGT
GGTTTCACCTCAAATCAAAGAAGAACTGTTTGCTATTACAAAAATAGAAG
ATACAGTGGAGAACTTTCATGCACAAGCAGAGAAACATTTTCATTACCAG
CAACTTGAATTTGGCTCCTTTTTACTTAGTAGGATGTTGCTAAATTTTTG
TTCAATTTTTGATTTATCTTCAGAGTTCATTTTAGCTTCCAAAGGGTTAA
CTCATCTAAATGAACTGCTTATGGCTTGTAAAAGTCATAAAGAAACCACC
AGTGTTCATACTGACTGGTTACTGGATCTTTATGCTGGAAATATAAAGAC
ATTGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMEDGTPKHIIQMT
GFKMEEKEALVKLLLKLDCTFIKSEKYKNCTHLIAERLCKSEKFLAACAA
GKWILTKDYIIHSAKSGRWLDETTYEWGYKIEKDSRYSPQMQSAPKRWRE
ELKRTGAPGAFHRWKVVLLVRTDKRSDSLIRVLEAGKANVILPKSSPSGI
THVIASNARIKAEKEKDNFKAPFYPIQYLGDFLLEKEIQNDEDSQTNSVW
TEHSNEETNKDFRKDAGFLEMKGALRETMYRTQKEMQNHEDVNVGSILIQ
HHKKEKFSGSSKDLKFVKMRNTFGSHTYENQKEIKKKDEDIQRSYTLRRK
RKKGKESNCKKGVEHEKIKSTLRRHIYNRDQKEMKNSIFAEYAKESKAMA
IKTDVDVVEIKNTLRKHIYRAQAVRYNCIRIDKQPVYNVEVKNAEFPRGV
LNLIESLIEGHFFKEAIEELSTLQAHYIPPVCVLHALLENVLQDNIDTFS
GRYFHILSALLHLHPPWKSPAMSRYYLELFQCPTCMKGAWSLVEVLIRSC
LFNESFCHQISENIGSKVLHLTLLKFFFNLIESEVQHLSQKLYDWSDSQN
LKITGKAMLLEIFWSGSETSGLLTKPVNMLLEWTIYSHKEKFKSNDVFKH
ELAYLLAGILGAAIDYWIFLGLKMGRNVMRHMSDDLGSYVSLSCDDFSSQ
ELEIFICSFSSSWLQMFVAEAVFKKLCLQSSGSVSSEPLSLQKMVYSYLS
ALGKTGVLGSGKIQVSKKIGQRPCFDSQRTLLMLNGTKQKQVEGLPELLD
LNLAKCSSSLKKLKKKSEGELSCSKENCPSVVKKMNFHKTNLKGETALHR
ACINNQVEKLILLLSLPGIDINVKDNAGWTPLHEACNYGNTVCVQEILQR
CPEVDLLTQVDGVTPLHDALSNGHVEIGKLLLQHGGPVLLQQRNAKGELP
LDYVVSPQIKEELFAITKIEDTVENFHAQAEKHFHYQQLEFGSFLLSRML
LNFCSIFDLSSEFILASKGLTHLNELLMACKSHKETTSVHTDWLLDLYAG
NIKTL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU5227
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human YBCP1 with N-terminal GST (contains preScission protease site); ; Estimated MW of expressed protein : 143802 Daltons