cDNA Clone YBCP1

• Plasmid: pGEX6P-3-YBCP1
• Parent Plasmid: pGEX6P3
• Expressed: GST-YBCP1
• Genbank: pGEX6P-3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGGAAGATGGTACCCCAAAGCATATCATCCAGATGACAGGATTTAAGATGGAAGAAAAAGAAGCGCTAGTCAAATTACTTTTAAAACTAGATTGCACTTTTATTAAGAGTGAGAAATACAAAAATTGTACACATCTTATAGCTGAACGCCTATGTAAGAGTGAAAAATTTTTAGCAGCTTGTGCGGCAGGAAAGTGGATACTAACCAAGGACTATATAATTCATAGTGCCAAAAGTGGCAGATGGCTTGATGAAACAACTTATGAATGGGGATATAAAATTGAAAAAGATTCCCGTTAT
TCACCTCAAATGCAATCTGCACCTAAAAGATGGCGTGAAGAACTGAAACG
CACTGGTGCTCCAGGAGCCTTCCACAGATGGAAAGTTGTCCTCCTTGTTA
GAACTGATAAGCGAAGCGATTCTCTTATAAGAGTTTTGGAGGCTGGAAAG
GCAAATGTTATTTTACCAAAAAGTTCACCAAGTGGAATAACTCATGTGAT
TGCCAGTAATGCAAGAATTAAAGCTGAGAAAGAAAAAGATAACTTTAAGG
CTCCATTTTATCCAATTCAGTATCTAGGGGATTTTCTTTTAGAGAAAGAA
ATTCAGAATGATGAAGATTCCCAAACCAATTCTGTTTGGACTGAACATAG
CAATGAAGAAACAAACAAAGATTTCAGGAAAGATGCAGGATTTCTTGAAA
TGAAAGGTGCCTTAAGAGAGACCATGTATAGAACCCAGAAAGAAATGCAA
AATCATGAAGATGTTAATGTTGGTTCTATTTTGATTCAACATCACAAAAA
AGAAAAATTCAGTGGTTCCAGTAAAGATTTGAAATTTGTTAAAATGAGAA
ATACCTTTGGAAGCCATACATATGAAAATCAGAAGGAAATTAAGAAAAAA
GATGAAGATATTCAGAGGAGTTATACTTTGAGGAGAAAACGCAAGAAAGG
AAAAGAAAGCAATTGCAAGAAAGGCGTTGAACATGAAAAAATAAAAAGTA
CCTTAAGAAGGCACATATATAATAGAGATCAGAAAGAAATGAAGAATTCT
ATTTTTGCTGAATATGCCAAAGAATCAAAAGCCATGGCTATTAAGACAGA
TGTGGATGTTGTTGAAATAAAAAATACCTTAAGGAAGCACATATATAGAG
CTCAGGCTGTCAGATACAACTGCATTAGAATAGATAAACAACCAGTGTAC
AACGTAGAGGTTAAAAATGCTGAATTTCCCAGAGGTGTATTAAATTTAAT
TGAAAGCCTCATAGAAGGACATTTTTTTAAAGAAGCAATTGAGGAACTTT
CCACTTTGCAGGCACATTATATCCCTCCTGTATGCGTTCTTCATGCCCTT
CTAGAGAACGTTCTACAGGATAACATAGATACATTTTCTGGTCGATACTT
TCATATATTGTCAGCTCTTCTTCACCTGCATCCTCCTTGGAAGTCTCCAG
CCATGTCGAGATATTATTTAGAGTTGTTTCAGTGTCCAACTTGTATGAAA
GGAGCATGGTCTTTAGTAGAAGTCCTTATCAGGTCTTGCCTTTTCAATGA
AAGCTTTTGTCATCAAATTTCAGAAAATATTGGCTCCAAGGTGCTCCACC
TGACGCTACTCAAATTTTTCTTTAATTTAATTGAAAGTGAAGTACAACAT
CTGAGTCAAAAGTTGTATGACTGGTCAGATTCTCAGAATCTGAAAATAAC
AGGAAAGGCAATGCTTCTTGAAATTTTTTGGTCAGGAAGTGAAACCTCTG
GGCTTTTGACCAAACCAGTAAATATGCTTTTGGAATGGACTATATATTCT
CACAAGGAAAAATTCAAGTCTAATGATGTCTTCAAACATGAACTAGCTTA
CTTATTGGCTGGAATTCTTGGAGCAGCAATAGATTATTGGATTTTCCTTG
GTCTTAAGATGGGTAGAAATGTGATGCGACACATGTCTGATGACTTAGGA
AGTTATGTTTCTCTTTCGTGTGATGACTTTTCTTCACAGGAATTAGAGAT
TTTCATTTGCTCCTTTTCCTCCTCCTGGCTTCAAATGTTTGTTGCAGAGG
CAGTCTTTAAAAAGTTGTGTCTACAGAGCTCTGGCAGTGTTTCTTCTGAG
CCACTCTCTCTTCAGAAAATGGTATATTCCTATTTATCAGCCTTGGGGAA
AACTGGTGTGCTTGGGTCTGGAAAGATTCAGGTGTCAAAGAAAATAGGAC
AGCGGCCTTGTTTTGACTCTCAGAGAACCTTACTAATGCTGAATGGTACT
AAACAAAAACAAGTCGAAGGGCTGCCAGAGTTACTAGACCTGAACCTTGC
TAAATGTTCCTCATCATTAAAAAAATTGAAAAAGAAGTCAGAAGGAGAAT
TGTCATGTTCCAAGGAGAATTGCCCCTCTGTAGTTAAAAAGATGAATTTT
CACAAGACTAATCTAAAAGGAGAAACAGCCCTGCATAGAGCTTGCATAAA
TAACCAAGTGGAGAAATTGATTCTTCTTCTCTCTTTGCCAGGAATAGACA
TCAATGTTAAAGACAATGCTGGCTGGACGCCTTTGCATGAAGCCTGTAAC
TATGGCAACACAGTGTGTGTCCAGGAAATTTTGCAACGTTGTCCAGAGGT
AGATCTGCTCACTCAAGTGGACGGGGTGACTCCTTTGCATGATGCACTGT
CAAACGGACATGTAGAAATTGGCAAGCTGCTACTACAGCATGGGGGCCCA
GTGCTTTTACAACAGAGGAATGCTAAGGGAGAATTGCCCTTGGATTATGT
GGTTTCACCTCAAATCAAAGAAGAACTGTTTGCTATTACAAAAATAGAAG
ATACAGTGGAGAACTTTCATGCACAAGCAGAGAAACATTTTCATTACCAG
CAACTTGAATTTGGCTCCTTTTTACTTAGTAGGATGTTGCTAAATTTTTG
TTCAATTTTTGATTTATCTTCAGAGTTCATTTTAGCTTCCAAAGGGTTAA
CTCATCTAAATGAACTGCTTATGGCTTGTAAAAGTCATAAAGAAACCACC
AGTGTTCATACTGACTGGTTACTGGATCTTTATGCTGGAAATATAAAGAC
ATTGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEDGTPKHIIQMTGFKMEE
KEALVKLLLKLDCTFIKSEKYKNCTHLIAERLCKSEKFLAACAAGKWILT
KDYIIHSAKSGRWLDETTYEWGYKIEKDSRYSPQMQSAPKRWREELKRTG
APGAFHRWKVVLLVRTDKRSDSLIRVLEAGKANVILPKSSPSGITHVIAS
NARIKAEKEKDNFKAPFYPIQYLGDFLLEKEIQNDEDSQTNSVWTEHSNE
ETNKDFRKDAGFLEMKGALRETMYRTQKEMQNHEDVNVGSILIQHHKKEK
FSGSSKDLKFVKMRNTFGSHTYENQKEIKKKDEDIQRSYTLRRKRKKGKE
SNCKKGVEHEKIKSTLRRHIYNRDQKEMKNSIFAEYAKESKAMAIKTDVD
VVEIKNTLRKHIYRAQAVRYNCIRIDKQPVYNVEVKNAEFPRGVLNLIES
LIEGHFFKEAIEELSTLQAHYIPPVCVLHALLENVLQDNIDTFSGRYFHI
LSALLHLHPPWKSPAMSRYYLELFQCPTCMKGAWSLVEVLIRSCLFNESF
CHQISENIGSKVLHLTLLKFFFNLIESEVQHLSQKLYDWSDSQNLKITGK
AMLLEIFWSGSETSGLLTKPVNMLLEWTIYSHKEKFKSNDVFKHELAYLL
AGILGAAIDYWIFLGLKMGRNVMRHMSDDLGSYVSLSCDDFSSQELEIFI
CSFSSSWLQMFVAEAVFKKLCLQSSGSVSSEPLSLQKMVYSYLSALGKTG
VLGSGKIQVSKKIGQRPCFDSQRTLLMLNGTKQKQVEGLPELLDLNLAKC
SSSLKKLKKKSEGELSCSKENCPSVVKKMNFHKTNLKGETALHRACINNQ
VEKLILLLSLPGIDINVKDNAGWTPLHEACNYGNTVCVQEILQRCPEVDL
LTQVDGVTPLHDALSNGHVEIGKLLLQHGGPVLLQQRNAKGELPLDYVVS
PQIKEELFAITKIEDTVENFHAQAEKHFHYQQLEFGSFLLSRMLLNFCSI
FDLSSEFILASKGLTHLNELLMACKSHKETTSVHTDWLLDLYAGNIKTL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU5226
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion of human YBCP1.; N.B. Sequence differences compared with John Rouse YBCP1 sequence: ; t417c (silent S139), c1518a (silent V506).; ; Estimated MW of expressed protein : 143192 Daltons